UniProt Accession

 RAIA_ECOLI; P0AD49; P11285 

Genbank Protein ID

 M10431; Z70523; U00096; AP009048; M58024 

Genbank Nucleotide ID

 AAA24328.1; CAA94436.1; AAC75646.1; BAA16481.1; AAA62782.1 

Protein Name

 Ribosome-associated inhibitor A 

Protein Synonyms/Alias

 Protein Y; SpotY; pY 

Gene Name

 raiA 

Gene Synonyms/Alias

 yfiA; b2597; JW2578 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
29	DRLAKLEKWQTHLIN	acetylation	[1]
43	NPHIILSKEPQGFVA	acetylation	[1]
66	GVLVASGKHEDMYTA	acetylation	[2]
80	AINELINKLERQLNK	acetylation	[1]
87	KLERQLNKLQHKGEA	acetylation	[1]
91	QLNKLQHKGEARRAA	acetylation	[3]
102	RRAATSVKDANFVEE	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 During stationary phase, prevents 70S dimer formation, probably in order to regulate translation efficiency during transition between the exponential and the stationary phases. In addition, during environmental stress such as cold shock or excessive cell density at stationary phase, stabilizes the 70S ribosome against dissociation, inhibits translation initiation and increase translation accuracy. When normal growth conditions are restored, is quickly released from the ribosome. Inhibits translation initiation by blocking the A-site (aminoacyl-tRNA site) and P-site (peptidyl-tRNA site) of the ribosome. Counteracts miscoding (translation errors) particularly efficiently at magnesium concentrations close to those observed in vivo but less efficiently at higher concentrations. Counteraction of miscoding was shown to be stronger than inhibition of translation, suggesting that the former activity could be the main function of RaiA in vivo. 

Sequence Annotation

 REGION 23 26 Interaction with rRNA (Potential).
 REGION 83 91 Interaction with rRNA (Potential).
 MOD_RES 66 66 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Reference proteome; Stress response; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 113 AA 

Protein Sequence

Gene Ontology

 GO:0022627; C:cytosolic small ribosomal subunit; IDA:EcoliWiki.
 GO:0043024; F:ribosomal small subunit binding; IDA:EcoCyc.
 GO:0045900; P:negative regulation of translational elongation; IDA:EcoCyc.
 GO:0045947; P:negative regulation of translational initiation; IDA:EcoCyc.
 GO:0044238; P:primary metabolic process; IEA:InterPro.
 GO:0009409; P:response to cold; IEP:EcoCyc. 

Interpro

 IPR003489; Ribosomal_S30Ae/sigma54_mod. 

Pfam

 PF02482; Ribosomal_S30AE 

SMART

  

PROSITE

  

PRINTS