UniProt Accession

 MDHC_RAT; O88989; O88585; Q6PCV2 

Genbank Protein ID

 AF093773; BC059124; AF075574 

Genbank Nucleotide ID

 AAC64180.1; AAH59124.1; AAC26799.1 

Protein Name

 Malate dehydrogenase, cytoplasmic 

Protein Synonyms/Alias

 Cytosolic malate dehydrogenase 

Gene Name

 Mdh1 

Gene Synonyms/Alias

 Mdh 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
103	MERKDLLKANVKIFK	acetylation	[1]
107	DLLKANVKIFKSQGA	acetylation	[1]
110	KANVKIFKSQGAALE	acetylation	[1]
118	SQGAALEKYAKKSVK	acetylation	[1]
118	SQGAALEKYAKKSVK	ubiquitination	[2]
164	RLDHNRAKSQIALKL	acetylation	[1]
164	RLDHNRAKSQIALKL	ubiquitination	[2]
179	GVTADDVKNVIIWGN	acetylation	[1]
199	YPDVNHAKVKLQGKE	acetylation	[1]
205	AKVKLQGKEVGVYEA	acetylation	[1]
205	AKVKLQGKEVGVYEA	ubiquitination	[2]
214	VGVYEALKDDSWLKG	acetylation	[1]
220	LKDDSWLKGEFITTV	acetylation	[1]
236	QRGAAVIKARKLSSA	acetylation	[1]
295	FPVVIKNKTWKFVEG	acetylation	[1]
298	VIKNKTWKFVEGLPI	acetylation	[1]
312	INDFSREKMDLTAKE	acetylation	[1]
318	EKMDLTAKELTEEKE	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

  

Sequence Annotation

 NP_BIND 11 17 NAD (By similarity).
 NP_BIND 129 131 NAD (By similarity).
 ACT_SITE 187 187 Proton acceptor (By similarity).
 BINDING 92 92 Substrate (By similarity).
 BINDING 98 98 Substrate (By similarity).
 BINDING 105 105 NAD (By similarity).
 BINDING 112 112 NAD (By similarity).
 BINDING 131 131 Substrate (By similarity).
 BINDING 162 162 Substrate (By similarity).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 118 118 N6-acetyllysine (By similarity).
 MOD_RES 121 121 N6-acetyllysine (By similarity).
 MOD_RES 210 210 Phosphotyrosine (By similarity).
 MOD_RES 241 241 Phosphoserine (By similarity).
 MOD_RES 298 298 N6-acetyllysine (By similarity).
 MOD_RES 333 333 Phosphoserine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 334 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0030060; F:L-malate dehydrogenase activity; IDA:RGD.
 GO:0051287; F:NAD binding; IDA:RGD.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006108; P:malate metabolic process; IDA:RGD.
 GO:0006734; P:NADH metabolic process; IDA:RGD.
 GO:0006107; P:oxaloacetate metabolic process; IDA:RGD.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. 

Interpro

 IPR001557; L-lactate/malate_DH.
 IPR022383; Lactate/malate_DH_C.
 IPR001236; Lactate/malate_DH_N.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR001252; Malate_DH_AS.
 IPR011274; Malate_DH_NAD-dep_euk.
 IPR010945; Malate_DH_type2.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02866; Ldh_1_C
 PF00056; Ldh_1_N 

SMART

  

PROSITE

 PS00068; MDH 

PRINTS