CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004564
UniProt Accession
Genbank Protein ID
 X52625 
Genbank Nucleotide ID
Protein Name
 Hydroxymethylglutaryl-CoA synthase, cytoplasmic 
Protein Synonyms/Alias
 HMG-CoA synthase; 3-hydroxy-3-methylglutaryl coenzyme A synthase 
Gene Name
 Hmgcs1 
Gene Synonyms/Alias
 Hmgcs 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
46YDGVDAGKYTIGLGQacetylation[1]
100GTETIIDKSKSVKSNacetylation[1]
206QHAYDFYKPDMLSEYacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. 
Sequence Annotation
 ACT_SITE 129 129 Potential.
 MOD_RES 4 4 Phosphoserine (By similarity).
 MOD_RES 46 46 N6-acetyllysine (By similarity).
 MOD_RES 273 273 N6-acetyllysine (By similarity).
 MOD_RES 495 495 Phosphoserine (By similarity).  
Keyword
 Acetylation; Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Cytoplasm; Direct protein sequencing; Lipid biosynthesis; Lipid metabolism; Phosphoprotein; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 520 AA 
Protein Sequence
MPGSLPLNAE ACWPKDVGIV ALEIYFPSQY VDQAELEKYD GVDAGKYTIG LGQARMGFCT 60
DREDINSLCL TVVQKLMERN SLSYDCIGRL EVGTETIIDK SKSVKSNLMQ LFEESGNTDI 120
EGIDTTNACY GGTAAVFNAV NWIESSSWDG RYALVVAGDI AIYASGNARP TGGVGAVALL 180
IGPNAPVIFD RGLRGTHMQH AYDFYKPDML SEYPVVDGKL SIQCYLSALD RCYSVYRKKI 240
RAQWQKEGKD KDFTLNDFGF MIFHSPYCKL VQKSLARMFL NDFLNDQNRD KNSIYSGLEA 300
FGDVKLEDTY FDRDVEKAFM KASAELFNQK TKASLLVSNQ NGNMYTSSVY GSLASVLAQY 360
SPQQLAGKRI GVFSYGSGLA ATLYSLKVTQ DATPGSALDK ITASLCDLKS RLDSRTCVAP 420
DVFAENMKLR EDTHHLANYI PQCSIDSLFE GTWYLVRVDE KHRRTYARRP STNDHSLDEG 480
VGLVHSNTAT EHIPSPAKKV PRLPATSGEP ESAVISNGEH 520 
Gene Ontology
 GO:0005829; C:cytosol; IDA:RGD.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0008144; F:drug binding; IDA:RGD.
 GO:0004421; F:hydroxymethylglutaryl-CoA synthase activity; IDA:RGD.
 GO:0016853; F:isomerase activity; IDA:RGD.
 GO:0043177; F:organic acid binding; IDA:RGD.
 GO:0042803; F:protein homodimerization activity; IDA:RGD.
 GO:0007420; P:brain development; IEP:RGD.
 GO:0071397; P:cellular response to cholesterol; IEP:RGD.
 GO:0071372; P:cellular response to follicle-stimulating hormone stimulus; IEP:RGD.
 GO:0006695; P:cholesterol biosynthetic process; IEA:UniProtKB-KW.
 GO:0008299; P:isoprenoid biosynthetic process; IEA:InterPro.
 GO:0001889; P:liver development; IEP:RGD.
 GO:0008584; P:male gonad development; IEP:RGD.
 GO:0001101; P:response to acid; IEP:RGD.
 GO:0042493; P:response to drug; IEP:RGD.
 GO:0055094; P:response to lipoprotein particle stimulus; IEP:RGD.
 GO:0009645; P:response to low light intensity stimulus; IEP:RGD.
 GO:0014074; P:response to purine-containing compound; IEP:RGD.
 GO:0046690; P:response to tellurium ion; IEP:RGD.
 GO:0033197; P:response to vitamin E; IEP:RGD. 
Interpro
 IPR000590; HMG_CoA_synt_AS.
 IPR013746; HMG_CoA_synt_C.
 IPR013528; HMG_CoA_synth_N.
 IPR010122; HMG_CoA_synthase_euk.
 IPR016039; Thiolase-like.
 IPR016038; Thiolase-like_subgr. 
Pfam
 PF08540; HMG_CoA_synt_C
 PF01154; HMG_CoA_synt_N 
SMART
  
PROSITE
 PS01226; HMG_COA_SYNTHASE 
PRINTS