CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023847
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Unconventional myosin-Va 
Protein Synonyms/Alias
 Dilute myosin heavy chain, non-muscle; Myosin heavy chain 12; Myosin-12; Myoxin 
Gene Name
 MYO5A 
Gene Synonyms/Alias
 MYH12 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9AASELYTKFARVWIPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation. 
Sequence Annotation
 DOMAIN 1 765 Myosin head-like.
 DOMAIN 766 788 IQ 1.
 DOMAIN 789 818 IQ 2.
 DOMAIN 814 836 IQ 3.
 DOMAIN 837 861 IQ 4.
 DOMAIN 862 883 IQ 5.
 DOMAIN 885 914 IQ 6.
 DOMAIN 1534 1810 Dilute.
 NP_BIND 163 170 ATP (Potential).
 REGION 643 665 Actin-binding (Potential).
 MOD_RES 600 600 Phosphoserine (By similarity).
 MOD_RES 1032 1032 Phosphothreonine (By similarity).
 MOD_RES 1452 1452 Phosphoserine.
 MOD_RES 1760 1760 Phosphothreonine (Potential).  
Keyword
 Actin-binding; Alternative splicing; ATP-binding; Calmodulin-binding; Coiled coil; Complete proteome; Motor protein; Myosin; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1855 AA 
Protein Sequence
MAASELYTKF ARVWIPDPEE VWKSAELLKD YKPGDKVLLL HLEEGKDLEY HLDPKTKELP 60
HLRNPDILVG ENDLTALSYL HEPAVLHNLR VRFIDSKLIY TYCGIVLVAI NPYEQLPIYG 120
EDIINAYSGQ NMGDMDPHIF AVAEEAYKQM ARDERNQSII VSGESGAGKT VSAKYAMRYF 180
ATVSGSASEA NVEEKVLASN PIMESIGNAK TTRNDNSSRF GKYIEIGFDK RYRIIGANMR 240
TYLLEKSRVV FQAEEERNYH IFYQLCASAK LPEFKMLRLG NADNFNYTKQ GGSPVIEGVD 300
DAKEMAHTRQ ACTLLGISES HQMGIFRILA GILHLGNVGF TSRDADSCTI PPKHEPLCIF 360
CELMGVDYEE MCHWLCHRKL ATATETYIKP ISKLQATNAR DALAKHIYAK LFNWIVDNVN 420
QALHSAVKQH SFIGVLDIYG FETFEINSFE QFCINYANEK LQQQFNMHVF KLEQEEYMKE 480
QIPWTLIDFY DNQPCINLIE SKLGILDLLD EECKMPKGTD DTWAQKLYNT HLNKCALFEK 540
PRLSNKAFII QHFADKVEYQ CEGFLEKNKD TVFEEQIKVL KSSKFKMLPE LFQDDEKAIS 600
PTSATSSGRT PLTRTPAKPT KGRPGQMAKE HKKTVGHQFR NSLHLLMETL NATTPHYVRC 660
IKPNDFKFPF TFDEKRAVQQ LRACGVLETI RISAAGFPSR WTYQEFFSRY RVLMKQKDVL 720
SDRKQTCKNV LEKLILDKDK YQFGKTKIFF RAGQVAYLEK LRADKLRAAC IRIQKTIRGW 780
LLRKKYLRMR KAAITMQRYV RGYQARCYAK FLRRTKAATI IQKYWRMYVV RRRYKIRRAA 840
TIVLQSYLRG FLARNRYRKI LREHKAVIIQ KRVRGWLART HYKRSMHAII YLQCCFRRMM 900
AKRELKKLKI EARSVERYKK LHIGMENKIM QLQRKVDEQN KDYKCLVEKL TNLEGIYNSE 960
TEKLRSDLER LQLSEEEAKV ATGRVLSLQE EIAKLRKDLE QTRSEKKCIE EHADRYKQET 1020
EQLVSNLKEE NTLLKQEKEA LNHRIVQQAK EMTETMEKKL VEETKQLELD LNDERLRYQN 1080
LLNEFSRLEE RYDDLKEEMT LMVHVPKPGH KRTDSTHSSN ESEYIFSSEI AEMEDIPSRT 1140
EEPSEKKVPL DMSLFLKLQK RVTELEQEKQ VMQDELDRKE EQVLRSKAKE EERPQIRGAE 1200
LEYESLKRQE LESENKKLKN ELNELRKALS EKSAPEVTAP GAPAYRVLME QLTSVSEELD 1260
VRKEEVLILR SQLVSQKEAI QPKDDKNTMT DSTILLEDVQ KMKDKGEIAQ AYIGLKETNR 1320
SSALDYHELN EDGELWLVYE GLKQANRLLE SQLQSQKRSH ENEAEALRGE IQSLKEENNR 1380
QQQLLAQNLQ LPPEARIEAS LQHEITRLTN ENLYFEELYA DDPKKYQSYR ISLYKRMIDL 1440
MEQLEKQDKT VRKLKKQLKV FAKKIGELEV GQMENISPGQ IIDEPIRPVN IPRKEKDFQG 1500
MLEYKKEDEQ KLVKNLILEL KPRGVAVNLI PGLPAYILFM CVRHADYLND DQKVRSLLTS 1560
TINSIKKVLK KRGDDFETVS FWLSNTCRFL HCLKQYSGEE GFMKHNTSRQ NEHCLTNFDL 1620
AEYRQVLSDL AIQIYQQLVR VLENILQPMI VSGMLEHETI QGVSGVKPTG LRKRTSSIAD 1680
EGTYTLDSIL RQLNSFHSVM CQHGMDPELI KQVVKQMFYI IGAITLNNLL LRKDMCSWSK 1740
GMQIRYNVSQ LEEWLRDKNL MNSGAKETLE PLIQAAQLLQ VKKKTDDDAE AICSMCNALT 1800
TAQIVKVLNL YTPVNEFEER VSVSFIRTIQ MRLRDRKDSP QLLMDAKHIF PVTFPFNPSS 1860
LALETIQIPA SLGLGFISRV 1880 
Gene Ontology
 GO:0042641; C:actomyosin; IEA:Compara.
 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0030426; C:growth cone; NAS:UniProtKB.
 GO:0032593; C:insulin-responsive compartment; ISS:UniProtKB.
 GO:0005882; C:intermediate filament; IEA:Compara.
 GO:0042470; C:melanosome; IEA:Compara.
 GO:0035371; C:microtubule plus end; IEA:Compara.
 GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0001750; C:photoreceptor outer segment; IEA:Compara.
 GO:0001726; C:ruffle; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; IEA:Compara.
 GO:0000146; F:microfilament motor activity; NAS:UniProtKB.
 GO:0030048; P:actin filament-based movement; NAS:UniProtKB.
 GO:0042640; P:anagen; IEA:Compara.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0032869; P:cellular response to insulin stimulus; ISS:UniProtKB.
 GO:0051643; P:endoplasmic reticulum localization; IEA:Compara.
 GO:0006887; P:exocytosis; IEA:Compara.
 GO:0030073; P:insulin secretion; IEA:Compara.
 GO:0031987; P:locomotion involved in locomotory behavior; IEA:Compara.
 GO:0042759; P:long-chain fatty acid biosynthetic process; IEA:Compara.
 GO:0042438; P:melanin biosynthetic process; IEA:Compara.
 GO:0030318; P:melanocyte differentiation; IEA:Compara.
 GO:0032402; P:melanosome transport; IEA:Compara.
 GO:0042552; P:myelination; IEA:Compara.
 GO:0042476; P:odontogenesis; IEA:Compara.
 GO:0072659; P:protein localization to plasma membrane; ISS:UniProtKB.
 GO:0031585; P:regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity; IEA:Compara.
 GO:0032252; P:secretory granule localization; IEA:Compara.
 GO:0050808; P:synapse organization; IEA:Compara.
 GO:0007268; P:synaptic transmission; IEA:Compara.
 GO:0016192; P:vesicle-mediated transport; ISS:UniProtKB.
 GO:0007601; P:visual perception; IEA:Compara. 
Interpro
 IPR018444; Dil_domain.
 IPR002710; Dilute.
 IPR000048; IQ_motif_EF-hand-BS.
 IPR001609; Myosin_head_motor_dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF01843; DIL
 PF00612; IQ
 PF00063; Myosin_head 
SMART
 SM00015; IQ
 SM00242; MYSc 
PROSITE
 PS51126; DILUTE
 PS50096; IQ 
PRINTS
 PR00193; MYOSINHEAVY.