CPLM-012200

Genbank Nucleotide ID

Nuclear receptor coactivator 4

Protein Synonyms/Alias

NCoA-4; Androgen receptor coactivator 70 kDa protein; 70 kDa AR-activator; 70 kDa androgen receptor coactivator; Androgen receptor-associated protein of 70 kDa; Ret-activating protein ELE1

Homo sapiens (Human)

Position	Peptide	Type	References
42	LRAEQQIKDNLREVK	ubiquitination	[1, 2, 3, 4]
49	KDNLREVKAQIHSCI	ubiquitination	[2, 3, 4]
181	NIGPFLEKRGCISMP	ubiquitination	[1, 3]
231	PQDWLTQKQTLENSQ	ubiquitination	[5]
263	GLENWLLKSEKSSYQ	ubiquitination	[1, 3, 5]
287	SFSIEMEKVGDQELP	ubiquitination	[3]
313	VTPQESHKLRKPENG	ubiquitination	[1, 5]
329	RETSEKFKLLFQSYN	ubiquitination	[3]
356	NCQGNQPKGVEIENL	ubiquitination	[3]
367	IENLGNLKCLNDHLE	ubiquitination	[3]
377	NDHLEAKKPLSTPSM	ubiquitination	[3]
424	VCDENCEKEALYKWL	ubiquitination	[3]
429	CEKEALYKWLLKKEG	ubiquitination	[3, 6, 7]
439	LKKEGKDKNGMPVEP	ubiquitination	[5]
465	NMWLCPRKEVIEQTK	ubiquitination	[3]
472	KEVIEQTKAPKAMTP	ubiquitination	[1, 3, 5]
475	IEQTKAPKAMTPSRI	ubiquitination	[3]
490	ADSFQVIKNSPLSEW	ubiquitination	[1, 3]
509	PYKEGSPKEVPGTED	ubiquitination	[3]
520	GTEDRAGKQKFKSPM	ubiquitination	[3]
544	ADWVLPGKKMGNLSQ	ubiquitination	[3]
545	DWVLPGKKMGNLSQL	ubiquitination	[3]
558	QLSSGEDKWLLRKKA	ubiquitination	[1, 3]
603	CMQLKVDKEKWLYRT	ubiquitination	[3]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma.

3D-structure; Activator; Alternative splicing; Chromosomal rearrangement; Complete proteome; Polymorphism; Proto-oncogene; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005634; C:nucleus; TAS:ProtInc.
GO:0050681; F:androgen receptor binding; NAS:UniProtKB.
GO:0003713; F:transcription coactivator activity; NAS:UniProtKB.
GO:0030521; P:androgen receptor signaling pathway; NAS:UniProtKB.
GO:0008584; P:male gonad development; TAS:ProtInc.
GO:0045893; P:positive regulation of transcription, DNA-dependent; NAS:UniProtKB.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.