CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031043
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA polymerase alpha subunit B 
Protein Synonyms/Alias
 cDNA FLJ60312, highly similar to DNA polymerase subunit alpha B 
Gene Name
 POLA2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
15IREVLTCKIEELGSEubiquitination[1, 2]
24EELGSELKEHYKIEAubiquitination[1]
56IGCDSNGKLNNKSVIubiquitination[2]
60SNGKLNNKSVILEGDubiquitination[1, 2, 3]
195SPFEDIFKQCLRTIIubiquitination[1, 4]
292DRFSRILKHILTQRSubiquitination[1, 2, 5, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 361 AA 
Protein Sequence
MFQKLPDIRE VLTCKIEELG SELKEHYKIE AFTPLLAPAQ EPVTLLGQIG CDSNGKLNNK 60
SVILEGDREH SSGAQIPVDL SELKEYSLFP GQVVIMEGIN TTGRKLVATK LYEGVPLPFY 120
QPTEEDADFE QSMVLVACGP YTTSDSITYD PLLDLIAVIN HDRPDVCILF GPFLDAKHEQ 180
VENCLLTSPF EDIFKQCLRT IIEGTRSSGS HLVFVPSLRD VHHEPVYPQP PFSYSDLSRE 240
DKKQVQFVSE PCSLSINGVI FGLTSTDLLF HLGAEEISSS SGTSDRFSRI LKHILTQRSY 300
YPLYPPQEDM AIDYESFYVY AQLPVTPDVL IIPSELRYFV KVGLNSAFSQ KHQNPVFDFP 360
F 361 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
 GO:0006260; P:DNA replication; IEA:InterPro. 
Interpro
 IPR007185; DNA_pol_alpha/epsilon_bsu.
 IPR016722; DNA_pol_alpha_bsu.
 IPR013627; Pol_alpha_B_N. 
Pfam
 PF04042; DNA_pol_E_B
 PF08418; Pol_alpha_B_N 
SMART
  
PROSITE
  
PRINTS