CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043660
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Putative ATP-dependent RNA helicase DHX30 
Protein Synonyms/Alias
  
Gene Name
 DHX30 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
20RASRDLLKEFPQPKNubiquitination[1]
110NELFDAAKYRVLADRubiquitination[2]
248HTVGTKTKLSTLTLLubiquitination[2]
329VPEPILRKIETFLNHubiquitination[2]
372LSDPITGKPYVPLLEubiquitination[2]
591PGFMYPVKEHYLEDIubiquitination[2]
601YLEDILAKLGKHQYLacetylation[3]
604DILAKLGKHQYLHRHubiquitination[2]
654LPGWQEIKGVQQRLQubiquitination[2, 4]
734ERYDLKTKVSCLETVubiquitination[2]
774FPRSRLEKMVPFQVPubiquitination[2]
796ENLVLQAKIHMPEKTubiquitination[2]
802AKIHMPEKTAVEFLSubiquitination[2]
810TAVEFLSKAVDSPNIubiquitination[2]
897RAEVDKVKALLSHDSubiquitination[2, 5]
1012GKVTRQGKFKPNSVTubiquitination[2]
1014VTRQGKFKPNSVTYRubiquitination[2]
1023NSVTYRTKSGNILLHubiquitination[2, 5]
1031SGNILLHKSTINREAubiquitination[2, 5]
1111SRTVRLLKELRRALGubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1166 AA 
Protein Sequence
MCVNPTPGGT ISRASRDLLK EFPQPKNLLN SVIGRALGIS HAKDKLVYVH TNGPKKKKVT 60
LHIKWPKSVE VEGYGSKKID AERQAAAAAC QLFKGWGLLG PRNELFDAAK YRVLADRFGS 120
PADSWWRPEP TMPPTSWRQL NPESIRPGGP GGLSRSLGRE EEEDEEEELE EGTIDVTDFL 180
SMTQQDSHAP LRDSRGSSFE MTDDDSAIRA LTQFPLPKNL LAKVIQIATS SSTAKNLMQF 240
HTVGTKTKLS TLTLLWPCPM TFVAKGRRKA EAENKAAALA CKKLKSLGLV DRNNEPLTHA 300
MYNLASLREL GETQRRPCTI QVPEPILRKI ETFLNHYPVE SSWIAPELRL QSDDILPLGK 360
DSGPLSDPIT GKPYVPLLEA EEVRLSQSLL ELWRRRGPVW QEAPQLPVDP HRDTILNAIE 420
QHPVVVISGD TGCGKTTRIP QLLLERYVTE GRGARCNVII TQPRRISAVS VAQRVSHELG 480
PSLRRNVGFQ VRLESKPPSR GGALLFCTVG ILLRKLQSNP SLEGVSHVIV DEVHERDVNT 540
DFLLILLKGL QRLNPALRLV LMSATGDNER FSRYFGGCPV IKVPGFMYPV KEHYLEDILA 600
KLGKHQYLHR HRHHESEDEC ALDLDLVTDL VLHIDARGEP GGILCFLPGW QEIKGVQQRL 660
QEALGMHESK YLILPVHSNI PMMDQKAIFQ QPPVGVRKIV LATNIAETSI TINDIVHVVD 720
SGLHKEERYD LKTKVSCLET VWVSRANVIQ RRGRAGRCQS GFAYHLFPRS RLEKMVPFQV 780
PEILRTPLEN LVLQAKIHMP EKTAVEFLSK AVDSPNIKAV DEAVILLQEI GVLDQREYLT 840
TLGQRLAHIS TDPRLAKAIV LAAIFRCLHP LLVVVSCLTR DPFSSSLQNR AEVDKVKALL 900
SHDSGSDHLA FVRAVAGWEE VLRWQDRSSR ENYLEENLLY APSLRFIHGL IKQFSENIYE 960
AFLVGKPSDC TLASAQCNEY SEEEELVKGV LMAGLYPNLI QVRQGKVTRQ GKFKPNSVTY 1020
RTKSGNILLH KSTINREATR LRSRWLTYFM AVKSNGSVFV RDSSQVHPLA VLLLTDGDVH 1080
IRDDGRRATI SLSDSDLLRL EGDSRTVRLL KELRRALGRM VERSLRSELA ALPPSVQEEH 1140
GQLLALLAEL LRGPCGSFDV RKTADD 1166 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR002464; DNA/RNA_helicase_DEAH_CS.
 IPR011709; DUF1605.
 IPR007502; Helicase-assoc_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00270; DEAD
 PF04408; HA2
 PF00271; Helicase_C
 PF07717; OB_NTP_bind 
SMART
 SM00487; DEXDc
 SM00847; HA2
 SM00490; HELICc 
PROSITE
 PS00690; DEAH_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS