UniProt Accession

 HES1_HUMAN; Q14469; Q6FHB2 

Genbank Protein ID

 L19314; AF264785; AK000415; CR541843 

Genbank Nucleotide ID

 AAA65220.1; AAF73060.1; BAA91149.1; CAG46641.1 

Protein Name

 Transcription factor HES-1 

Protein Synonyms/Alias

 Class B basic helix-loop-helix protein 39; bHLHb39; Hairy and enhancer of split 1; Hairy homolog; Hairy-like protein; hHL 

Gene Name

 HES1 

Gene Synonyms/Alias

 BHLHB39; HL; HRY 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
86	DILEMTVKHLRNLQR	ubiquitination	[1, 2]
109	TDPSVLGKYRAGFSE	ubiquitination	[1, 2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage. 

Sequence Annotation

 DOMAIN 34 91 bHLH.
 DOMAIN 110 143 Orange.
 MOTIF 275 278 WRPW motif.  

Keyword

 Complete proteome; DNA-binding; Nucleus; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 280 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0071820; F:N-box binding; ISS:UniProtKB.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0001078; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; ISS:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
 GO:0008134; F:transcription factor binding; NAS:UniProtKB.
 GO:0021984; P:adenohypophysis development; IEA:Compara.
 GO:0035910; P:ascending aorta morphogenesis; ISS:BHF-UCL.
 GO:0042668; P:auditory receptor cell fate determination; IEA:Compara.
 GO:0061309; P:cardiac neural crest cell development involved in outflow tract morphogenesis; ISS:BHF-UCL.
 GO:0007155; P:cell adhesion; IEA:Compara.
 GO:0048469; P:cell maturation; IEA:Compara.
 GO:0016477; P:cell migration; IEA:Compara.
 GO:0048667; P:cell morphogenesis involved in neuron differentiation; IEA:Compara.
 GO:0090102; P:cochlea development; IEA:Compara.
 GO:0072049; P:comma-shaped body morphogenesis; IEA:Compara.
 GO:0061009; P:common bile duct development; IEA:Compara.
 GO:0003143; P:embryonic heart tube morphogenesis; ISS:BHF-UCL.
 GO:0031018; P:endocrine pancreas development; TAS:Reactome.
 GO:0021861; P:forebrain radial glial cell differentiation; ISS:UniProtKB.
 GO:0072012; P:glomerulus vasculature development; IEA:Compara.
 GO:0021575; P:hindbrain morphogenesis; IEA:Compara.
 GO:0060716; P:labyrinthine layer blood vessel development; IEA:Compara.
 GO:0046331; P:lateral inhibition; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0030324; P:lung development; IEA:Compara.
 GO:0072282; P:metanephric nephron tubule morphogenesis; IEA:Compara.
 GO:0030901; P:midbrain development; IEA:Compara.
 GO:0021555; P:midbrain-hindbrain boundary morphogenesis; IEA:Compara.
 GO:0045608; P:negative regulation of auditory receptor cell differentiation; IEA:Compara.
 GO:2000978; P:negative regulation of forebrain neuron differentiation; ISS:UniProtKB.
 GO:0060253; P:negative regulation of glial cell proliferation; ISS:UniProtKB.
 GO:0048715; P:negative regulation of oligodendrocyte differentiation; ISS:UniProtKB.
 GO:2000227; P:negative regulation of pancreatic A cell differentiation; IEA:Compara.
 GO:2000974; P:negative regulation of pro-B cell differentiation; IMP:UniProtKB.
 GO:2000737; P:negative regulation of stem cell differentiation; IMP:UniProtKB.
 GO:0061106; P:negative regulation of stomach neuroendocrine cell differentiation; IEA:Compara.
 GO:0097150; P:neuronal stem cell maintenance; IEP:UniProtKB.
 GO:0007219; P:Notch signaling pathway; IDA:UniProtKB.
 GO:0021557; P:oculomotor nerve development; IEA:Compara.
 GO:0060037; P:pharyngeal system development; ISS:BHF-UCL.
 GO:0048711; P:positive regulation of astrocyte differentiation; ISS:UniProtKB.
 GO:0030513; P:positive regulation of BMP signaling pathway; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; ISS:BHF-UCL.
 GO:0043388; P:positive regulation of DNA binding; ISS:UniProtKB.
 GO:0045977; P:positive regulation of mitotic cell cycle, embryonic; ISS:BHF-UCL.
 GO:0045747; P:positive regulation of Notch signaling pathway; IEA:Compara.
 GO:0042102; P:positive regulation of T cell proliferation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; ISS:BHF-UCL.
 GO:0042517; P:positive regulation of tyrosine phosphorylation of Stat3 protein; ISS:UniProtKB.
 GO:0006461; P:protein complex assembly; ISS:UniProtKB.
 GO:0045598; P:regulation of fat cell differentiation; IEA:Compara.
 GO:0003266; P:regulation of secondary heart field cardioblast proliferation; ISS:BHF-UCL.
 GO:0060164; P:regulation of timing of neuron differentiation; IEA:Compara.
 GO:0072141; P:renal interstitial cell development; IEA:Compara.
 GO:0072050; P:S-shaped body morphogenesis; IEA:Compara.
 GO:0007224; P:smoothened signaling pathway; IEA:Compara.
 GO:0035019; P:somatic stem cell maintenance; IEA:Compara.
 GO:0007262; P:STAT protein import into nucleus; ISS:UniProtKB.
 GO:0021537; P:telencephalon development; IEA:Compara.
 GO:0048538; P:thymus development; ISS:BHF-UCL.
 GO:0021558; P:trochlear nerve development; IEA:Compara.
 GO:0060675; P:ureteric bud morphogenesis; IEA:Compara.
 GO:0097084; P:vascular smooth muscle cell development; ISS:BHF-UCL.
 GO:0060412; P:ventricular septum morphogenesis; ISS:BHF-UCL. 

Interpro

 IPR011598; bHLH_dom.
 IPR003650; Orange.
 IPR018352; Orange_subgr. 

Pfam

 PF07527; Hairy_orange
 PF00010; HLH 

SMART

 SM00353; HLH
 SM00511; ORANGE 

PROSITE

 PS50888; BHLH
 PS51054; ORANGE 

PRINTS