CPLM-023128

Genbank Nucleotide ID

CCR4-NOT transcription complex subunit 11

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
319	WDKSMCVKNSTGVEI	ubiquitination	[1]
327	NSTGVEIKRIMAKAF	ubiquitination	[1, 2]
332	EIKRIMAKAFKSPLS	ubiquitination	[1]
335	RIMAKAFKSPLSSPQ	ubiquitination	[1, 2, 3, 4]
352	QLLGELEKDPKLVYH	ubiquitination	[1, 2, 5]
495	AGLFRLLKTLDTGET	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
507	GETPSETKMSK****	ubiquitination	[1, 2, 5, 6, 8]
510	PSETKMSK*******	ubiquitination	[1, 5]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[8] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]

Functional Description

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is required for the association of CNOT10 with the CCR4-NOT complex. Seems not to be required for complex deadenylase function.

Complete proteome; Cytoplasm; Nucleus; Reference proteome; RNA-mediated gene silencing; Transcription; Transcription regulation; Translation regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0030014; C:CCR4-NOT complex; IDA:UniProtKB.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0008283; P:cell proliferation; NAS:UniProtKB.
GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.