UniProt Accession

 VRK2_HUMAN; Q86Y07; B4DKL0; D6W5D4; D6W5D6; Q49AK9; Q53EU9; Q53S39; Q53S77; Q53TU1; Q86Y08; Q86Y09; Q86Y10; Q86Y11; Q86Y12; Q8IXI5; Q99987 

Genbank Protein ID

 AB000450; AJ512204; AY228367; AY228368; AY228369; AY228370; AY228371; AY228372; AK296611; AK223540; AC007250; AC068193; AC073215; CH471053; CH471053; CH471053; CH471053; CH471053; BC027854 

Genbank Nucleotide ID

 BAA19109.1; CAD54446.2; AAO73047.1; AAO73048.1; AAO73049.1; AAO73050.1; AAO73051.1; AAO73052.1; BAG59222.1; BAD97260.1; AAY15019.1; AAX93262.1; AAY14648.1; EAX00062.1; EAX00064.1; EAX00066.1; EAX00067.1; EAX00068.1; AAH27854.1 

Protein Name

 Serine/threonine-protein kinase VRK2 

Protein Synonyms/Alias

 Vaccinia-related kinase 2 

Gene Name

 VRK2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
10	PKRNEKYKLPIPFPE	ubiquitination	[1]
33	GNQWVLGKKIGSGGF	ubiquitination	[2]
75	GPLFSELKFYQRVAK	ubiquitination	[2]
112	GSGLTEFKGRSYRFM	ubiquitination	[1, 3]
177	ANLLLGYKNPDQVYL	ubiquitination	[2, 3, 4]
200	YCPNGNHKQYQENPR	ubiquitination	[3]
223	FTSLDAHKGVALSRR	ubiquitination	[2]
255	LPWEQNLKDPVAVQT	ubiquitination	[2, 4]
264	PVAVQTAKTNLLDEL	ubiquitination	[2, 4]
328	GPLDFSTKGQSINVH	ubiquitination	[2, 3, 4]
383	WKVQKEEKLIGLMNN	ubiquitination	[2]
402	ESTRRRQKYQESQEP	ubiquitination	[2]
419	EVNSFPQKISYTQFP	ubiquitination	[4]
443	FTSPDIFKKSRSPSW	ubiquitination	[2, 3, 4]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant. 

Sequence Annotation

 DOMAIN 29 319 Protein kinase.
 NP_BIND 35 43 ATP (By similarity).
 REGION 397 508 Interaction with MAP3K7.
 ACT_SITE 166 166 Proton acceptor (By similarity).
 BINDING 61 61 ATP (By similarity).
 MOD_RES 336 336 Phosphothreonine.  

Keyword

 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Endoplasmic reticulum; Host-virus interaction; Kinase; Membrane; Mitochondrion; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 508 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031966; C:mitochondrial membrane; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0034599; P:cellular response to oxidative stress; IMP:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB.
 GO:2000659; P:regulation of interleukin-1-mediated signaling pathway; IMP:UniProtKB.
 GO:0043408; P:regulation of MAPK cascade; IMP:UniProtKB.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

  

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS