UniProt Accession

 PNPO_MOUSE; Q91XF0; Q3TP70; Q3U445; Q3U4X1 

Genbank Protein ID

 AK154004; AK154443; AK164667; AL596384; BC010785; BC026564 

Genbank Nucleotide ID

 BAE32309.1; BAE32590.1; BAE37867.1; CAM21902.1; AAH10785.1; AAH26564.1 

Protein Name

 Pyridoxine-5'-phosphate oxidase 

Protein Synonyms/Alias

 Pyridoxamine-phosphate oxidase 

Gene Name

 Pnpo 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
100	SARMLLLKGFGKDGF	acetylation	[1, 2]
100	SARMLLLKGFGKDGF	succinylation	[2]
100	SARMLLLKGFGKDGF	ubiquitination	[3]
104	LLLKGFGKDGFRFFT	acetylation	[4]
104	LLLKGFGKDGFRFFT	ubiquitination	[3]
152	PVKKLPEKEAENYFH	ubiquitination	[3]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379] 

Functional Description

 Catalyzes the oxidation of either pyridoxine 5'- phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP) (By similarity). 

Sequence Annotation

 NP_BIND 110 111 FMN (By similarity).
 NP_BIND 174 175 FMN (By similarity).
 REGION 225 227 Substrate binding (By similarity).
 BINDING 95 95 FMN (By similarity).
 BINDING 98 98 FMN; via amide nitrogen (By similarity).
 BINDING 100 100 Substrate (By similarity).
 BINDING 117 117 FMN (By similarity).
 BINDING 157 157 Substrate (By similarity).
 BINDING 161 161 Substrate (By similarity).
 BINDING 165 165 Substrate (By similarity).
 MOD_RES 165 165 Phosphoserine.
 MOD_RES 241 241 Phosphoserine.  

Keyword

 Complete proteome; Direct protein sequencing; Flavoprotein; FMN; Oxidoreductase; Phosphoprotein; Pyridoxal phosphate; Pyridoxine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 261 AA 

Protein Sequence

Gene Ontology

 GO:0010181; F:FMN binding; IEA:InterPro.
 GO:0004733; F:pyridoxamine-phosphate oxidase activity; IEA:EC.
 GO:0008615; P:pyridoxine biosynthetic process; IEA:UniProtKB-KW. 

Interpro

 IPR000659; Pyridox_Oxase.
 IPR019740; Pyridox_Oxase_CS.
 IPR011576; Pyridox_Oxase_FMN-bd.
 IPR019576; Pyridoxamine_oxidase_dimer_C.
 IPR012349; Split_barrel_FMN-bd. 

Pfam

 PF10590; PNPOx_C
 PF01243; Pyridox_oxidase 

SMART

  

PROSITE

 PS01064; PYRIDOX_OXIDASE 

PRINTS