UniProt Accession

 CAF20_YEAST; P12962; D6W2X6 

Genbank Protein ID

 X15731; X89633; Z75184; L22433; BK006948 

Genbank Nucleotide ID

 CAA33752.1; CAA61782.1; CAA99501.1; AAA16544.1; DAA11042.1 

Protein Name

 Cap-associated protein CAF20 

Protein Synonyms/Alias

 20 kDa cap-associated protein; CCR4-associated factor 2; p20 

Gene Name

 CAF20 

Gene Synonyms/Alias

 CAF2; CAP20; YOR276W; O5453W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
89	CTFEAKKKGSGEDDE	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Acts as an inhibitor of cap-dependent translation. Competes with eIF4G1/TIF4631 and EAP1 for binding to eIF4E/TIF45 and interferes with the formation of the eIF4F complex, inhibiting translation and stabilizing mRNA. Binding affinity for eIF4E/TIF45 is 10-fold less than that of eIF4G1/TIF4631. Required for induction of pseudohyphal growth in response to nitrogen limitation, probably by regulating STE12 translation. 

Sequence Annotation

 MOD_RES 78 78 Phosphoserine.
 MOD_RES 91 91 Phosphoserine.
 MOD_RES 99 99 Phosphothreonine.
 MOD_RES 101 101 Phosphothreonine.
 MOD_RES 102 102 Phosphothreonine.
 MOD_RES 154 154 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Protein synthesis inhibitor; Reference proteome; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 161 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005845; C:mRNA cap binding complex; IPI:SGD.
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
 GO:0045182; F:translation regulator activity; IPI:SGD.
 GO:0017148; P:negative regulation of translation; IMP:SGD.
 GO:0010606; P:positive regulation of cytoplasmic mRNA processing body assembly; IMP:SGD. 

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