UniProt Accession

 RNAS1_BOVIN; P61823; A6QPW8; P00656; Q9TSF2 

Genbank Protein ID

 X07283; BC149529; BC149636; S80747; S81740 

Genbank Nucleotide ID

 CAA30263.1; AAI49530.1; AAI49637.1; AAB35594.1; AAB36134.1 

Protein Name

 Ribonuclease pancreatic 

Protein Synonyms/Alias

 RNase 1; RNase A 

Gene Name

 RNASE1 

Gene Synonyms/Alias

 RNS1 

Created Date

 July 27, 2013 

Organism

 Bos taurus (Bovine) 

NCBI Taxa ID

 9913 

Lysine Modification

Position	Peptide	Type	References
4	****MALKSLVLLSL	acetylation	[1]
27	RVQPSLGKETAAAKF	acetylation	[1]
27	RVQPSLGKETAAAKF	glycation	[2, 3]
33	GKETAAAKFERQHMD	acetylation	[1]
33	GKETAAAKFERQHMD	glycation	[2, 3, 4]
57	NYCNQMMKSRNLTKD	acetylation	[1]
57	NYCNQMMKSRNLTKD	glycation	[4]
63	MKSRNLTKDRCKPVN	acetylation	[1]
63	MKSRNLTKDRCKPVN	glycation	[2, 3]
67	NLTKDRCKPVNTFVH	acetylation	[1]
67	NLTKDRCKPVNTFVH	glycation	[2, 3, 4]
87	VQAVCSQKNVACKNG	acetylation	[1]
92	SQKNVACKNGQTNCY	acetylation	[1]
92	SQKNVACKNGQTNCY	glycation	[5]
117	CRETGSSKYPNCAYK	acetylation	[1]
117	CRETGSSKYPNCAYK	glycation	[4, 5]
124	KYPNCAYKTTQANKH	acetylation	[1]
124	KYPNCAYKTTQANKH	glycation	[4, 5]
130	YKTTQANKHIIVACE	acetylation	[1]
130	YKTTQANKHIIVACE	glycation	[4, 5]

Reference

 [1] The role of lysine in the action of bovine pancreatic ribonuclease A.
 Walter B, Wold F.
 Biochemistry. 1976 Jan 27;15(2):304-10. [PMID: 942854]
 [2] Glycation of amino groups in protein. Studies on the specificity of modification of RNase by glucose.
 Watkins NG, Thorpe SR, Baynes JW.
 J Biol Chem. 1985 Sep 5;260(19):10629-36. [PMID: 4030761]
 [3] Proteomic analysis of the site specificity of glycation and carboxymethylation of ribonuclease.
 Brock JW, Hinton DJ, Cotham WE, Metz TO, Thorpe SR, Baynes JW, Ames JM.
 J Proteome Res. 2003 Sep-Oct;2(5):506-13. [PMID: 14582647]
 [4] Enrichment and analysis of nonenzymatically glycated peptides: boronate affinity chromatography coupled with electron-transfer dissociation mass spectrometry.
 Zhang Q, Tang N, Brock JW, Mottaz HM, Ames JM, Baynes JW, Smith RD, Metz TO.
 J Proteome Res. 2007 Jun;6(6):2323-30. [PMID: 17488106]
 [5] Identification of glucose-derived cross-linking sites in ribonuclease A.
 Dai Z, Wang B, Sun G, Fan X, Anderson VE, Monnier VM.
 J Proteome Res. 2008 Jul;7(7):2756-68. [PMID: 18500835] 

Functional Description

 Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA. 

Sequence Annotation

 REGION 67 71 Substrate binding.
 ACT_SITE 38 38 Proton acceptor.
 ACT_SITE 145 145 Proton donor.
 BINDING 33 33 Substrate.
 BINDING 36 36 Substrate.
 BINDING 92 92 Substrate.
 BINDING 111 111 Substrate.
 CARBOHYD 27 27 N-linked (Glc) (glycation); in vitro.
 CARBOHYD 33 33 N-linked (Glc) (glycation); in vitro.
 CARBOHYD 60 60 N-linked (GlcNAc...); partial.
 CARBOHYD 63 63 N-linked (Glc) (glycation); in vitro.
 CARBOHYD 67 67 N-linked (Glc) (glycation); in vitro.
 DISULFID 52 110
 DISULFID 66 121
 DISULFID 84 136
 DISULFID 91 98  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Disulfide bond; Endonuclease; Glycation; Glycoprotein; Hydrolase; Nuclease; Reference proteome; Secreted; Signal. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 150 AA 

Protein Sequence

Gene Ontology

 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0004522; F:pancreatic ribonuclease activity; IEA:EC.
 GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC. 

Interpro

 IPR001427; RNaseA.
 IPR023411; RNaseA_AS.
 IPR023412; RNaseA_domain. 

Pfam

 PF00074; RnaseA 

SMART

 SM00092; RNAse_Pc 

PROSITE

 PS00127; RNASE_PANCREATIC 

PRINTS

 PR00794; RIBONUCLEASE.