UniProt Accession

 PGK_YEAST; P00560; D6VR21 

Genbank Protein ID

 J01342; X59720; M14438; K00553; BK006937 

Genbank Nucleotide ID

 AAA88729.1; CAA42329.2; AAA34864.1; AAA34863.1; DAA07490.1 

Protein Name

 Phosphoglycerate kinase 

Protein Synonyms/Alias

  

Gene Name

 PGK1 

Gene Synonyms/Alias

 YCR012W; YCR12W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
6	**MSLSSKLSVQDLD	acetylation	[1]
15	SVQDLDLKDKRVFIR	acetylation	[1]
32	FNVPLDGKKITSNQR	acetylation	[1]
48	VAALPTIKYVLEHHP	acetylation	[1]
48	VAALPTIKYVLEHHP	ubiquitination	[2]
74	PNGERNEKYSLAPVA	acetylation	[1]
74	PNGERNEKYSLAPVA	ubiquitination	[2]
82	YSLAPVAKELQSLLG	acetylation	[1]
82	YSLAPVAKELQSLLG	ubiquitination	[3]
142	GQKVKASKEDVQKFR	acetylation	[1]
147	ASKEDVQKFRHELSS	acetylation	[1]
190	AAGFLLEKELKYFGK	acetylation	[1]
190	AAGFLLEKELKYFGK	ubiquitination	[2]
193	FLLEKELKYFGKALE	acetylation	[1]
193	FLLEKELKYFGKALE	ubiquitination	[2]
197	KELKYFGKALENPTR	acetylation	[1]
197	KELKYFGKALENPTR	ubiquitination	[2, 3]
214	LAILGGAKVADKIQL	acetylation	[1]
214	LAILGGAKVADKIQL	ubiquitination	[2]
218	GGAKVADKIQLIDNL	acetylation	[1]
258	IGDSIFDKAGAEIVP	acetylation	[1]
258	IGDSIFDKAGAEIVP	ubiquitination	[2, 3, 4]
266	AGAEIVPKLMEKAKA	acetylation	[1]
266	AGAEIVPKLMEKAKA	ubiquitination	[2]
274	LMEKAKAKGVEVVLP	ubiquitination	[3]
302	NTKTVTDKEGIPAGW	acetylation	[1, 5]
302	NTKTVTDKEGIPAGW	ubiquitination	[2, 3]
320	DNGPESRKLFAATVA	acetylation	[1]
320	DNGPESRKLFAATVA	ubiquitination	[2]
345	PGVFEFEKFAAGTKA	ubiquitination	[2]
351	EKFAAGTKALLDEVV	acetylation	[1]
351	EKFAAGTKALLDEVV	ubiquitination	[2]
359	ALLDEVVKSSAAGNT	acetylation	[1]
379	GDTATVAKKYGVTDK	acetylation	[1]
379	GDTATVAKKYGVTDK	ubiquitination	[3]
380	DTATVAKKYGVTDKI	acetylation	[1]
380	DTATVAKKYGVTDKI	ubiquitination	[2]
386	KKYGVTDKISHVSTG	acetylation	[1]
415	GVAFLSEKK******	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [4] A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.
 Ziv I, Matiuhin Y, Kirkpatrick DS, Erpapazoglou Z, Leon S, Pantazopoulou M, Kim W, Gygi SP, Haguenauer-Tsapis R, Reis N, Glickman MH, Kleifeld O.
 Mol Cell Proteomics. 2011 May;10(5):M111.009753. [PMID: 21427232]
 [5] Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.
 Xie H, Bandhakavi S, Roe MR, Griffin TJ.
 J Proteome Res. 2007 May;6(5):2019-26. [PMID: 17397211] 

Functional Description

  

Sequence Annotation

 NP_BIND 371 374 ATP.
 REGION 24 26 Substrate binding.
 REGION 63 66 Substrate binding.
 BINDING 39 39 Substrate.
 BINDING 122 122 Substrate.
 BINDING 169 169 Substrate.
 BINDING 218 218 ATP.
 BINDING 311 311 ATP; via carbonyl oxygen.
 BINDING 335 335 ATP.
 BINDING 342 342 ATP.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 93 93 Phosphothreonine.
 MOD_RES 110 110 Phosphoserine.
 MOD_RES 130 130 Phosphoserine.
 MOD_RES 154 154 Phosphoserine.
 MOD_RES 172 172 Phosphoserine.
 MOD_RES 203 203 Phosphothreonine.
 MOD_RES 241 241 Phosphothreonine.
 MOD_RES 298 298 Phosphothreonine.
 MOD_RES 318 318 Phosphoserine.
 MOD_RES 331 331 Phosphothreonine.
 MOD_RES 392 392 Phosphothreonine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 416 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004618; F:phosphoglycerate kinase activity; IDA:SGD.
 GO:0006094; P:gluconeogenesis; IMP:SGD.
 GO:0006096; P:glycolysis; IMP:SGD. 

Interpro

 IPR001576; Phosphoglycerate_kinase.
 IPR015901; Phosphoglycerate_kinase_C.
 IPR015911; Phosphoglycerate_kinase_CS.
 IPR015824; Phosphoglycerate_kinase_N. 

Pfam

 PF00162; PGK 

SMART

  

PROSITE

 PS00111; PGLYCERATE_KINASE 

PRINTS

 PR00477; PHGLYCKINASE.