UniProt Accession

 KCRM_MOUSE; P07310 

Genbank Protein ID

 X03233 

Genbank Nucleotide ID

 CAA26979.1 

Protein Name

 Creatine kinase M-type 

Protein Synonyms/Alias

 Creatine kinase M chain; M-CK 

Gene Name

 Ckm 

Gene Synonyms/Alias

 Ckmm 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
9	PFGNTHNKFKLNYKP	acetylation	[1]
9	PFGNTHNKFKLNYKP	ubiquitination	[2]
11	GNTHNKFKLNYKPQE	acetylation	[1]
11	GNTHNKFKLNYKPQE	ubiquitination	[2]
15	NKFKLNYKPQEEYPD	acetylation	[1]
15	NKFKLNYKPQEEYPD	ubiquitination	[2]
25	EEYPDLSKHNNHMAK	ubiquitination	[2]
32	KHNNHMAKVLTPDLY	acetylation	[1]
32	KHNNHMAKVLTPDLY	ubiquitination	[2]
41	LTPDLYNKLRDKETP	acetylation	[1]
41	LTPDLYNKLRDKETP	ubiquitination	[2]
101	QDRHGGYKPTDKHKT	acetylation	[1]
105	GGYKPTDKHKTDLNH	ubiquitination	[2]
107	YKPTDKHKTDLNHEN	ubiquitination	[2]
116	DLNHENLKGGDDLDP	acetylation	[1]
116	DLNHENLKGGDDLDP	ubiquitination	[2]
138	VRTGRSIKGYTLPPH	ubiquitination	[2]
156	GERRAVEKLSVEALN	acetylation	[1]
156	GERRAVEKLSVEALN	ubiquitination	[2]
170	NSLTGEFKGKYYPLK	acetylation	[1]
170	NSLTGEFKGKYYPLK	ubiquitination	[2]
172	LTGEFKGKYYPLKSM	acetylation	[1]
196	DDHFLFDKPVSPLLL	acetylation	[1]
196	DDHFLFDKPVSPLLL	ubiquitination	[2]
223	GIWHNDNKSFLVWVN	ubiquitination	[2]
242	LRVISMEKGGNMKEV	acetylation	[1]
242	LRVISMEKGGNMKEV	ubiquitination	[2]
247	MEKGGNMKEVFRRFC	ubiquitination	[2]
259	RFCVGLQKIEEIFKK	acetylation	[1]
259	RFCVGLQKIEEIFKK	ubiquitination	[2]
265	QKIEEIFKKAGHPFM	acetylation	[1]
265	QKIEEIFKKAGHPFM	ubiquitination	[2]
266	KIEEIFKKAGHPFMW	ubiquitination	[2]
298	LRGGVHVKLANLSKH	acetylation	[1]
298	LRGGVHVKLANLSKH	ubiquitination	[2]
304	VKLANLSKHPKFEEI	acetylation	[1]
304	VKLANLSKHPKFEEI	ubiquitination	[2]
307	ANLSKHPKFEEILTR	acetylation	[1]
307	ANLSKHPKFEEILTR	ubiquitination	[2]
365	KLMVEMEKKLEKGQS	acetylation	[1]
369	EMEKKLEKGQSIDDM	ubiquitination	[2]

Reference

 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. 

Sequence Annotation

 DOMAIN 11 98 Phosphagen kinase N-terminal.
 DOMAIN 125 367 Phosphagen kinase C-terminal.
 NP_BIND 128 132 ATP (By similarity).
 NP_BIND 320 325 ATP (By similarity).
 BINDING 191 191 ATP (By similarity).
 BINDING 236 236 ATP (By similarity).
 BINDING 292 292 ATP (By similarity).
 BINDING 335 335 ATP (By similarity).  

Keyword

 ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 381 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004111; F:creatine kinase activity; IDA:MGI.
 GO:0046314; P:phosphocreatine biosynthetic process; IDA:MGI. 

Interpro

 IPR000749; ATP-guanido_PTrfase.
 IPR022415; ATP-guanido_PTrfase_AS.
 IPR022414; ATP-guanido_PTrfase_cat.
 IPR022413; ATP-guanido_PTrfase_N.
 IPR014746; Gln_synth/guanido_kin_cat_dom. 

Pfam

 PF00217; ATP-gua_Ptrans
 PF02807; ATP-gua_PtransN 

SMART

  

PROSITE

 PS00112; PHOSPHAGEN_KINASE
 PS51510; PHOSPHAGEN_KINASE_C
 PS51509; PHOSPHAGEN_KINASE_N 

PRINTS