CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020413
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ER degradation-enhancing alpha-mannosidase-like protein 3 
Protein Synonyms/Alias
 Alpha-1,2-mannosidase EDEM3 
Gene Name
 EDEM3 
Gene Synonyms/Alias
 C1orf22 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
189MAKQLGYKLLPAFNTubiquitination[1]
208PYPRINLKFGIRKPEubiquitination[1]
262ALDFLWEKRQRSSNLubiquitination[1, 2]
435TLIENLNKYARVPCGubiquitination[1, 3, 4]
447PCGFAAMKDVRTGSHubiquitination[1]
555PLKNVVDKSCPRGIIubiquitination[1]
594EHLEILKKMGVSLIHubiquitination[1]
705NPEAVMGKIALIQRGubiquitination[1]
719GQCMFAEKARNIQNAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Involved in endoplasmic reticulum-associated degradation (ERAD). Accelerates the glycoprotein ERAD by proteasomes. This process depends on mannose-trimming from the N-glycans. Seems to have alpha 1,2-mannosidase activity (By similarity). 
Sequence Annotation
 DOMAIN 674 779 PA.
 MOTIF 929 932 Prevents secretion from ER (Potential).
 ACT_SITE 146 146 Proton donor (By similarity).
 ACT_SITE 293 293 By similarity.
 ACT_SITE 405 405 By similarity.
 MOD_RES 303 303 Phosphotyrosine (By similarity).
 MOD_RES 310 310 Phosphoserine (By similarity).
 CARBOHYD 118 118 N-linked (GlcNAc...) (Potential).
 CARBOHYD 195 195 N-linked (GlcNAc...).
 CARBOHYD 504 504 N-linked (GlcNAc...) (Potential).
 CARBOHYD 511 511 N-linked (GlcNAc...) (Potential).
 CARBOHYD 810 810 N-linked (GlcNAc...) (Potential).
 CARBOHYD 814 814 N-linked (GlcNAc...) (Potential).
 CARBOHYD 900 900 N-linked (GlcNAc...) (Potential).  
Keyword
 Complete proteome; Endoplasmic reticulum; Glycoprotein; Hydrolase; Phosphoprotein; Polymorphism; Reference proteome; Signal; Unfolded protein response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 932 AA 
Protein Sequence
MSEAGGRGCG SPVPQRARWR LVAATAAFCL VSATSVWTAG AEPMSREEKQ KLGNQVLEMF 60
DHAYGNYMEH AYPADELMPL TCRGRVRGQE PSRGDVDDAL GKFSLTLIDS LDTLVVLNKT 120
KEFEDAVRKV LRDVNLDNDV VVSVFETNIR VLGGLLGGHS LAIMLKEKGE YMQWYNDELL 180
QMAKQLGYKL LPAFNTTSGL PYPRINLKFG IRKPEARTGT ETDTCTACAG TLILEFAALS 240
RFTGATIFEE YARKALDFLW EKRQRSSNLV GVTINIHTGD WVRKDSGVGA GIDSYYEYLL 300
KAYVLLGDDS FLERFNTHYD AIMRYISQPP LLLDVHIHKP MLNARTWMDA LLAFFPGLQV 360
LKGDIRPAIE THEMLYQVIK KHNFLPEAFT TDFRVHWAQH PLRPEFAEST YFLYKATGDP 420
YYLEVGKTLI ENLNKYARVP CGFAAMKDVR TGSHEDRMDS FFLAEMFKYL YLLFADKEDI 480
IFDIEDYIFT TEAHLLPLWL STTNQSISKK NTTSEYTELD DSNFDWTCPN TQILFPNDPL 540
YAQSIREPLK NVVDKSCPRG IIRVEESFRS GAKPPLRARD FMATNPEHLE ILKKMGVSLI 600
HLKDGRVQLV QHAIQAASSI DAEDGLRFMQ EMIELSSQQQ KEQQLPPRAV QIVSHPFFGR 660
VVLTAGPAQF GLDLSKHKET RGFVASSKPS NGCSELTNPE AVMGKIALIQ RGQCMFAEKA 720
RNIQNAGAIG GIVIDDNEGS SSDTAPLFQM AGDGKDTDDI KIPMLFLFSK EGSIILDAIR 780
EYEEVEVLLS DKAKDRDPEM ENEEQPSSEN DSQNQSGEQI SSSSQEVDLV DQESSEENSL 840
NSHPESLSLA DMDNAASISP SEQTSNPTEN HETTNLNGEC TDLDNQLQEQ SETEEDSNPN 900
VSWGKKVQPI DSILADWNED IEAFEMMEKD EL 932 
Gene Ontology
 GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0004569; F:glycoprotein endo-alpha-1,2-mannosidase activity; IEA:Compara.
 GO:0004571; F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity; IEA:EC.
 GO:0006516; P:glycoprotein catabolic process; IEA:Compara.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IEA:Compara.
 GO:0006457; P:protein folding; TAS:Reactome.
 GO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome.
 GO:0006986; P:response to unfolded protein; IEA:UniProtKB-KW. 
Interpro
 IPR001382; Glyco_hydro_47.
 IPR003137; Protease-assoc_domain. 
Pfam
 PF01532; Glyco_hydro_47
 PF02225; PA 
SMART
  
PROSITE
 PS00014; ER_TARGET 
PRINTS
 PR00747; GLYHDRLASE47.