CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013182
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Monofunctional C1-tetrahydrofolate synthase, mitochondrial 
Protein Synonyms/Alias
 Formyltetrahydrofolate synthetase 
Gene Name
 Mthfd1l 
Gene Synonyms/Alias
 Fthfsdc1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
188VTDINLGKLVRGDAPacetylation[1, 2, 3, 4]
188VTDINLGKLVRGDAPsuccinylation[4]
351RWRLHCLKLQPLSPVacetylation[1, 3, 4]
585TNDRFLRKITIGQGSacetylation[4]
585TNDRFLRKITIGQGSsuccinylation[4]
595IGQGSTEKGYSRQAQacetylation[3, 4]
595IGQGSTEKGYSRQAQsuccinylation[4]
726MEKFFNIKCRASGLVubiquitination[5]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2. 
Sequence Annotation
 NP_BIND 422 429 ATP (By similarity).
 REGION 32 347 Methylenetetrahydrofolate dehydrogenase
 REGION 348 977 Formyltetrahydrofolate synthetase.
 MOD_RES 188 188 N6-acetyllysine (By similarity).  
Keyword
 3D-structure; Acetylation; ATP-binding; Complete proteome; Ligase; Mitochondrion; Nucleotide-binding; One-carbon metabolism; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 977 AA 
Protein Sequence
MSVRLPLLLR QLGRQQLPSG PACRLRELCR SGSRSSSSGG GDPEGLRGRR LQDGQTFSSH 60
GPGNPEAPGM DSIVRDVIHN SKEVLSLLQE KNPAFKPVLV VIQAGDDNLM KDMNQNLAKE 120
AGLDITHICL PPDSGEDEII DEILKINEDP RVHGLTLQIS EDSLSNKVLN ALKPEKDVDG 180
VTDINLGKLV RGDAPECFVS PLAKAAVELV EKSGITLDGK KVLVVGAHGP LEAALQWLFQ 240
RKGSMTMSCP WATPQLPDKL READIVVLGS PKPEEIPAVW IPSGTTILNC FHDFLSGKLS 300
GGSPGVPVDK LIAEESVSLL AAALRIQNMV SSGRRWLREQ QHRRWRLHCL KLQPLSPVPS 360
DIEISRGQTP KAVDVLAKEI GLLADEIEIY GKSKAKVQLS LLERLKDQTD GKYVLVAGIT 420
PTPLGEGKST VTIGLVQALT AHLKVNSFAC LRQPSQGPTF GVKGGAAGGG YAQVIPMEEF 480
NLHLTGDIHA ITAANNLLAA AIDTRILHES TQTDKALYNR LVPLVNGVRE FSEIQLSRLK 540
KLGIHKTDPS TLTEEEVRKF ARLNIDPATI TWQRVLDTND RFLRKITIGQ GSTEKGYSRQ 600
AQFDIAVASE IMAVLALTDS LTDMKERLGR MVVASDKDGQ PVTAEDLGVT GALTVLMKDA 660
IKPNLMQTLE GTPVFVHAGP FANIAHGNSS VLADKIALKL VGEEGFVVTE AGFGADIGME 720
KFFNIKCRAS GLVPNVVVLV ATVRALKMHG GGPSVTAGVP LKKEYTEENI QLVADGCCNL 780
QKQIQIAQLF GVPVVVALNV FKTDTRAEID LVCELAKRAG AFDAVPCYHW SAGGKGSVDL 840
ARAVREAANK RSRFQFLYDV QLPIVEKIRV IAQTVYGAKD IELSPEAQSK IDRYTQQGFG 900
NLPICMAKTH LSLSHEPDKK GVPRDFTLPI SDVRASIGAG FIYPLVGTMS TMPGLPTRPC 960
FYDIDLDTET EQVKGLF 977 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004329; F:formate-tetrahydrofolate ligase activity; ISO:MGI.
 GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IEA:InterPro.
 GO:0009257; P:10-formyltetrahydrofolate biosynthetic process; ISO:MGI.
 GO:0006730; P:one-carbon metabolic process; IBA:RefGenome.
 GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway. 
Interpro
 IPR000559; Formate_THF_ligase.
 IPR020628; Formate_THF_ligase_CS.
 IPR016040; NAD(P)-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR000672; THF_DH/CycHdrlase.
 IPR020630; THF_DH/CycHdrlase_cat_dom.
 IPR020631; THF_DH/CycHdrlase_NAD-bd_dom. 
Pfam
 PF01268; FTHFS
 PF00763; THF_DHG_CYH
 PF02882; THF_DHG_CYH_C 
SMART
  
PROSITE
 PS00721; FTHFS_1
 PS00722; FTHFS_2 
PRINTS
 PR00085; THFDHDRGNASE.