CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022144
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RNA-binding protein 12 
Protein Synonyms/Alias
 RNA-binding motif protein 12; SH3/WW domain anchor protein in the nucleus; SWAN 
Gene Name
 RBM12 
Gene Synonyms/Alias
 KIAA0765; HRIHFB2091 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
336VDAVHLLKDHVGRNNubiquitination[1]
349NNGNGLVKFLSPQDTubiquitination[2]
444LPFEAENKHVIDFFKubiquitination[1, 3]
451KHVIDFFKKLDIVEDacetylation[4]
485FRNEADYKAALCRHKacetylation[3]
485FRNEADYKAALCRHKubiquitination[1, 3]
893VCLKYNEKGMPTGEAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
  
Sequence Annotation
 DOMAIN 304 379 RRM 1.
 DOMAIN 430 507 RRM 2.
 DOMAIN 856 932 RRM 3.
 MOD_RES 420 420 Phosphoserine.
 MOD_RES 422 422 Phosphoserine.
 MOD_RES 424 424 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 932 AA 
Protein Sequence
MAVVIRLQGL PIVAGTMDIR HFFSGLTIPD GGVHIVGGEL GEAFIVFATD EDARLGMMRT 60
GGTIKGSKVT LLLSSKTEMQ NMIELSRRRF ETANLDIPPA NASRSGPPPS SGMSSRVNLP 120
TTVSNFNNPS PSVVTATTSV HESNKNIQTF STASVGTAPP NMGASFGSPT FSSTVPSTAS 180
PMNTVPPPPI PPIPAMPSLP PMPSIPPIPV PPPVPTLPPV PPVPPIPPVP SVPPMTPLPP 240
MSGMPPLNPP PVAPLPAGMN GSGAPMNLNN NLNPMFLGPL NPVNPIQMNS QSSVKPLPIN 300
PDDLYVSVHG MPFSAMENDV RDFFHGLRVD AVHLLKDHVG RNNGNGLVKF LSPQDTFEAL 360
KRNRMLMIQR YVEVSPATER QWVAAGGHIT FKQNMGPSGQ THPPPQTLPR SKSPSGQKRS 420
RSRSPHEAGF CVYLKGLPFE AENKHVIDFF KKLDIVEDSI YIAYGPNGKA TGEGFVEFRN 480
EADYKAALCR HKQYMGNRFI QVHPITKKGM LEKIDMIRKR LQNFSYDQRE MILNPEGDVN 540
SAKVCAHITN IPFSITKMDV LQFLEGIPVD ENAVHVLVDN NGQGLGQALV QFKNEDDARK 600
SERLHRKKLN GREAFVHVVT LEDMREIEKN PPAQGKKGLK MPVPGNPAVP GMPNAGLPGV 660
GLPSAGLPGA GLPSTGLPGS AITSAGLPGA GMPSAGIPSA GGEEHAFLTV GSKEANNGPP 720
FNFPGNFGGS NAFGPPIPPP GLGGGAFGDA RPGMPSVGNS GLPGLGLDVP GFGGGPNNLS 780
GPSGFGGGPQ NFGNGPGSLG GPPGFGSGPP GLGSAPGHLG GPPAFGPGPG PGPGPGPIHI 840
GGPPGFASSS GKPGPTVIKV QNMPFTVSID EILDFFYGYQ VIPGSVCLKY NEKGMPTGEA 900
MVAFESRDEA TAAVIDLNDR PIGSRKVKLV LG 932 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 
Pfam
  
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS