CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010632
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Proteasome activator complex subunit 2 
Protein Synonyms/Alias
 11S regulator complex subunit beta; REG-beta; Activator of multicatalytic protease subunit 2; Proteasome activator 28 subunit beta; PA28b; PA28beta 
Gene Name
 Psme2 
Gene Synonyms/Alias
 Pa28b1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
15RLSGEARKQVDVFRQacetylation[1, 2]
15RLSGEARKQVDVFRQubiquitination[3]
145FGVAIQEKVLERVNAubiquitination[3, 4, 5]
235VNPKGEEKPSMY***ubiquitination[5]
Reference
 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
 Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 10 10 Phosphoserine (By similarity).  
Keyword
 Acetylation; Complete proteome; Phosphoprotein; Proteasome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 239 AA 
Protein Sequence
MAKPCGVRLS GEARKQVDVF RQNLFQEADD FLCTFLPRKI ISLSQLLQED SLNVADLSSL 60
RAPLDIPIPD PPPKDDEMET DKQEKKEVPK CGYLPGNEKL LALLALVKPE VWTLKEKCIL 120
VITWIQHLIP KIEDGNDFGV AIQEKVLERV NAVKTKVEAF QTTISKYFSE RGDAVAKASK 180
DTHVMDYRAL VHERDEAAYG ALRAMVLDLR AFYAELYHII SSNLEKIVNP KGEEKPSMY 239 
Gene Ontology
 GO:0008537; C:proteasome activator complex; IEA:InterPro. 
Interpro
 IPR003185; Proteasome_activ_REG_asu.
 IPR009077; Proteasome_activ_REG_asu/bsu.
 IPR003186; Proteasome_activ_REG_bsu. 
Pfam
 PF02251; PA28_alpha
 PF02252; PA28_beta 
SMART
  
PROSITE
  
PRINTS