CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007217
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vacuolar protein 8 
Protein Synonyms/Alias
  
Gene Name
 VAC8 
Gene Synonyms/Alias
 YEB3; YEL013W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
77AFAEITEKYVRQVSRubiquitination[1, 2]
174GALIPLTKLAKSKHIubiquitination[2]
244VDEANRKKLAQTEPRubiquitination[2]
370ASSEKNRKEFFESGAubiquitination[2]
515DIINGVRKMADVTFEubiquitination[1, 2]
Reference
 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole. 
Sequence Annotation
 REPEAT 37 75 ARM 1.
 REPEAT 76 114 ARM 2.
 REPEAT 116 155 ARM 3.
 REPEAT 157 196 ARM 4.
 REPEAT 198 237 ARM 5.
 REPEAT 239 280 ARM 6.
 REPEAT 282 321 ARM 7.
 REPEAT 323 363 ARM 8.
 REPEAT 407 446 ARM 9.
 MOD_RES 11 11 Phosphoserine.
 MOD_RES 16 16 Phosphoserine.
 LIPID 2 2 N-myristoyl glycine.
 LIPID 4 4 S-palmitoyl cysteine (Probable).
 LIPID 5 5 S-palmitoyl cysteine (Probable).
 LIPID 7 7 S-palmitoyl cysteine (Probable).  
Keyword
 Complete proteome; Lipoprotein; Membrane; Myristate; Palmitate; Phosphoprotein; Reference proteome; Repeat; Vacuole. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 578 AA 
Protein Sequence
MGSCCSCLKD SSDEASVSPI ADNEREAVTL LLGYLEDKDQ LDFYSGGPLK ALTTLVYSDN 60
LNLQRSAALA FAEITEKYVR QVSREVLEPI LILLQSQDPQ IQVAACAALG NLAVNNENKL 120
LIVEMGGLEP LINQMMGDNV EVQCNAVGCI TNLATRDDNK HKIATSGALI PLTKLAKSKH 180
IRVQRNATGA LLNMTHSEEN RKELVNAGAV PVLVSLLSST DPDVQYYCTT ALSNIAVDEA 240
NRKKLAQTEP RLVSKLVSLM DSPSSRVKCQ ATLALRNLAS DTSYQLEIVR AGGLPHLVKL 300
IQSDSIPLVL ASVACIRNIS IHPLNEGLIV DAGFLKPLVR LLDYKDSEEI QCHAVSTLRN 360
LAASSEKNRK EFFESGAVEK CKELALDSPV SVQSEISACF AILALADVSK LDLLEANILD 420
ALIPMTFSQN QEVSGNAAAA LANLCSRVNN YTKIIEAWDR PNEGIRGFLI RFLKSDYATF 480
EHIALWTILQ LLESHNDKVE DLVKNDDDII NGVRKMADVT FERLQRSGID VKNPGSNNNP 540
SSNDNNSNNN DTGSEHQPVE DASLELYNIT QQILQFLH 578 
Gene Ontology
 GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
 GO:0071563; C:Myo2p-Vac17p-Vac8p transport complex; IDA:SGD.
 GO:0071561; C:nucleus-vacuole junction; IDA:SGD.
 GO:0043495; F:protein anchor; IMP:SGD.
 GO:0071255; P:CVT vesicle assembly; IMP:SGD.
 GO:0016236; P:macroautophagy; IMP:SGD.
 GO:0071562; P:nucleus-vacuole junction assembly; IMP:SGD.
 GO:0042144; P:vacuole fusion, non-autophagic; IMP:SGD.
 GO:0000011; P:vacuole inheritance; IMP:SGD. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000225; Armadillo.
 IPR026958; Vac8. 
Pfam
 PF00514; Arm 
SMART
 SM00185; ARM 
PROSITE
 PS50176; ARM_REPEAT 
PRINTS