CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022872
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha-aminoadipic semialdehyde synthase, mitochondrial 
Protein Synonyms/Alias
 LKR/SDH; Lysine ketoglutarate reductase; LKR; LOR; Saccharopine dehydrogenase; SDH 
Gene Name
 AASS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
539DICKQEEKLGFLVAKubiquitination[1]
707YPNRDSTKYAEIYGIacetylation[2]
893LDGEIGAKGLMGPFSubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Bifunctional enzyme that catalyzes the first two steps in lysine degradation. The N-terminal and the C-terminal contain lysine-ketoglutarate reductase and saccharopine dehydrogenase activity, respectively. 
Sequence Annotation
 REGION 33 455 Lysine-ketoglutarate reductase.
 REGION 477 926 Saccharopine dehydrogenase.  
Keyword
 Complete proteome; Mitochondrion; Multifunctional enzyme; NAD; NADP; Oxidoreductase; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 926 AA 
Protein Sequence
MLQVHRTGLG RLGVSLSKGL HHKAVLAVRR EDVNAWERRA PLAPKHIKGI TNLGYKVLIQ 60
PSNRRAIHDK DYVKAGGILQ EDISEACLIL GVKRPPEEKL MSRKTYAFFS HTIKAQEANM 120
GLLDEILKQE IRLIDYEKMV DHRGVRVVAF GQWAGVAGMI NILHGMGLRL LALGHHTPFM 180
HIGMAHNYRN SSQAVQAVRD AGYEISLGLM PKSIGPLTFV FTGTGNVSKG AQAIFNELPC 240
EYVEPHELKE VSQTGDLRKV YGTVLSRHHH LVRKTDAVYD PAEYDKHPER YISRFNTDIA 300
PYTTCLINGI YWEQNTPRLL TRQDAQSLLA PGKFSPAGVE GCPALPHKLV AICDISADTG 360
GSIEFMTECT TIEHPFCMYD ADQHIIHDSV EGSGILMCSI DNLPAQLPIE ATECFGDMLY 420
PYVEEMILSD ATQPLESQNF SPVVRDAVIT SNGTLPDKYK YIQTLRESRE RAQSLSMGTR 480
RKVLVLGSGY ISEPVLEYLS RDGNIEITVG SDMKNQIEQL GKKYNINPVS MDICKQEEKL 540
GFLVAKQDLV ISLLPYVLHP LVAKACITNK VNMVTASYIT PALKELEKSV EDAGITIIGE 600
LGLDPGLDHM LAMETIDKAK EVGATIESYI SYCGGLPAPE HSNNPLRYKF SWSPVGVLMN 660
VMQSATYLLD GKVVNVAGGI SFLDAVTSMD FFPGLNLEGY PNRDSTKYAE IYGISSAHTL 720
LRGTLRYKGY MKALNGFVKL GLINREALPA FRPEANPLTW KQLLCDLVGI SPSSEHDVLK 780
EAVLKKLGGD NTQLEAAEWL GLLGDEQVPQ AESILDALSK HLVMKLSYGP EEKDMIVMRD 840
SFGIRHPSGH LEHKTIDLVA YGDINGFSAM AKTVGLPTAM AAKMLLDGEI GAKGLMGPFS 900
KEIYGPILER IKAEGIIYTT QSTIKP 926 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0047131; F:saccharopine dehydrogenase (NAD+, L-glutamate-forming) activity; NAS:UniProtKB.
 GO:0047130; F:saccharopine dehydrogenase (NADP+, L-lysine-forming) activity; EXP:Reactome.
 GO:0034641; P:cellular nitrogen compound metabolic process; TAS:Reactome.
 GO:0033512; P:L-lysine catabolic process to acetyl-CoA via saccharopine; IEA:UniProtKB-UniPathway.
 GO:0006554; P:lysine catabolic process; NAS:UniProtKB.
 GO:0051262; P:protein tetramerization; TAS:UniProtKB. 
Interpro
 IPR007886; AlaDH/PNT_N.
 IPR007698; AlaDH/PNT_NAD(H)-bd.
 IPR016040; NAD(P)-bd_dom.
 IPR005097; Saccharopine_DH/HSpermid_syn. 
Pfam
 PF01262; AlaDh_PNT_C
 PF05222; AlaDh_PNT_N
 PF03435; Saccharop_dh 
SMART
 SM01002; AlaDh_PNT_C
 SM01003; AlaDh_PNT_N 
PROSITE
 PS00836; ALADH_PNT_1
 PS00837; ALADH_PNT_2 
PRINTS