CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008902
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha-N-acetylglucosaminidase 
Protein Synonyms/Alias
 N-acetyl-alpha-glucosaminidase; NAG; Alpha-N-acetylglucosaminidase 82 kDa form; Alpha-N-acetylglucosaminidase 77 kDa form 
Gene Name
 NAGLU 
Gene Synonyms/Alias
 UFHSD1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
59VERALAAKPGLDTYSubiquitination[1, 2, 3, 4]
586ARSAYLSKELASLLRubiquitination[2, 3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Involved in the degradation of heparan sulfate. 
Sequence Annotation
 CARBOHYD 261 261 N-linked (GlcNAc...) (Potential).
 CARBOHYD 272 272 N-linked (GlcNAc...) (Potential).
 CARBOHYD 435 435 N-linked (GlcNAc...) (Potential).
 CARBOHYD 503 503 N-linked (GlcNAc...) (Potential).
 CARBOHYD 526 526 N-linked (GlcNAc...) (Potential).
 CARBOHYD 532 532 N-linked (GlcNAc...).  
Keyword
 Complete proteome; Direct protein sequencing; Disease mutation; Glycoprotein; Glycosidase; Hydrolase; Lysosome; Mucopolysaccharidosis; Polymorphism; Reference proteome; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 743 AA 
Protein Sequence
MEAVAVAAAV GVLLLAGAGG AAGDEAREAA AVRALVARLL GPGPAADFSV SVERALAAKP 60
GLDTYSLGGG GAARVRVRGS TGVAAAAGLH RYLRDFCGCH VAWSGSQLRL PRPLPAVPGE 120
LTEATPNRYR YYQNVCTQSY SFVWWDWARW EREIDWMALN GINLALAWSG QEAIWQRVYL 180
ALGLTQAEIN EFFTGPAFLA WGRMGNLHTW DGPLPPSWHI KQLYLQHRVL DQMRSFGMTP 240
VLPAFAGHVP EAVTRVFPQV NVTKMGSWGH FNCSYSCSFL LAPEDPIFPI IGSLFLRELI 300
KEFGTDHIYG ADTFNEMQPP SSEPSYLAAA TTAVYEAMTA VDTEAVWLLQ GWLFQHQPQF 360
WGPAQIRAVL GAVPRGRLLV LDLFAESQPV YTRTASFQGQ PFIWCMLHNF GGNHGLFGAL 420
EAVNGGPEAA RLFPNSTMVG TGMAPEGISQ NEVVYSLMAE LGWRKDPVPD LAAWVTSFAA 480
RRYGVSHPDA GAAWRLLLRS VYNCSGEACR GHNRSPLVRR PSLQMNTSIW YNRSDVFEAW 540
RLLLTSAPSL ATSPAFRYDL LDLTRQAVQE LVSLYYEEAR SAYLSKELAS LLRAGGVLAY 600
ELLPALDEVL ASDSRFLLGS WLEQARAAAV SEAEADFYEQ NSRYQLTLWG PEGNILDYAN 660
KQLAGLVANY YTPRWRLFLE ALVDSVAQGI PFQQHQFDKN VFQLEQAFVL SKQRYPSQPR 720
GDTVDLAKKI FLKYYPRWVA GSW 743 
Gene Ontology
 GO:0043202; C:lysosomal lumen; TAS:Reactome.
 GO:0004561; F:alpha-N-acetylglucosaminidase activity; TAS:ProtInc.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0021680; P:cerebellar Purkinje cell layer development; IEA:Compara.
 GO:0006027; P:glycosaminoglycan catabolic process; TAS:Reactome.
 GO:0060119; P:inner ear receptor cell development; IEA:Compara.
 GO:0045475; P:locomotor rhythm; IEA:Compara.
 GO:0007040; P:lysosome organization; IEA:Compara.
 GO:0042474; P:middle ear morphogenesis; IEA:Compara.
 GO:0007399; P:nervous system development; TAS:ProtInc.
 GO:0046548; P:retinal rod cell development; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR007781; NAGLU.
 IPR024732; NAGLU_C.
 IPR024240; NAGLU_N.
 IPR024733; NAGLU_tim-barrel. 
Pfam
 PF05089; NAGLU
 PF12972; NAGLU_C
 PF12971; NAGLU_N 
SMART
  
PROSITE
  
PRINTS