UniProt Accession

 NEK7_HUMAN; Q8TDX7; A6NGT8 

Genbank Protein ID

 AB062450; CK819069; AC093419; AC093561; CH471067 

Genbank Nucleotide ID

 BAB85632.1; EAW91294.1 

Protein Name

 Serine/threonine-protein kinase Nek7 

Protein Synonyms/Alias

 Never in mitosis A-related kinase 7; NimA-related protein kinase 7 

Gene Name

 NEK7 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
20	VPQFQPQKALRPDMG	ubiquitination	[1]
63	DGVPVALKKVQIFDL	ubiquitination	[2]
64	GVPVALKKVQIFDLM	ubiquitination	[1]
74	IFDLMDAKARADCIK	ubiquitination	[1, 2]
81	KARADCIKEIDLLKQ	ubiquitination	[1, 3]
87	IKEIDLLKQLNHPNV	ubiquitination	[1, 3]
163	RVMHRDIKPANVFIT	ubiquitination	[1]
189	LGRFFSSKTTAAHSL	ubiquitination	[1, 2]
249	NLYSLCKKIEQCDYP	ubiquitination	[1]
281	CINPDPEKRPDVTYV	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1. 

Sequence Annotation

 DOMAIN 34 299 Protein kinase.
 NP_BIND 40 48 ATP.
 REGION 20 33 NTE motif.
 ACT_SITE 161 161 Proton acceptor (By similarity).
 BINDING 63 63 ATP.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 195 195 Phosphoserine; by NEK9.  

Keyword

 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Magnesium; Metal-binding; Microtubule; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 302 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; ISS:UniProtKB.
 GO:0000910; P:cytokinesis; TAS:UniProtKB.
 GO:0007346; P:regulation of mitotic cell cycle; TAS:UniProtKB.
 GO:0051225; P:spindle assembly; TAS:UniProtKB. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS

 PR00109; TYRKINASE.