CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030521
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein with KRAB and SCAN domains 1 
Protein Synonyms/Alias
 cDNA FLJ34152 fis, clone FCBBF3012901, highly similar to Zinc finger with KRAB and SCAN domain-containing protein 1 
Gene Name
 ZKSCAN1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
67ENEESTSKAETSEDSubiquitination[1, 2, 3]
162EEVPTGTKSHRCDECubiquitination[4]
171HRCDECGKCFTRSSSubiquitination[4]
183SSSLIRHKIIHTGEKubiquitination[4]
246QRIHSGEKPYECNECubiquitination[4, 5]
255YECNECGKAFSQSSDubiquitination[4, 5]
265SQSSDLTKHQRIHTGubiquitination[4, 5]
283YECSECGKAFNRNSYubiquitination[4, 6]
347DAFGAFLKSCV****ubiquitination[4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 350 AA 
Protein Sequence
MASALFTADS QAMVKIEDMA VSLILEEWGC QNLARRNLSR DNRQENYGSA FPQGGENRNE 60
NEESTSKAET SEDSASRGET TGRSQKEFGE KRDQEGKTGE RQQKNPEEKT RKEKRDSGPA 120
IGKDKKTITG ERGPREKGKG LGRSFSLSSN FTTPEEVPTG TKSHRCDECG KCFTRSSSLI 180
RHKIIHTGEK PYECSECGKA FSLNSNLVLH QRIHTGEKPH ECNECGKAFS HSSNLILHQR 240
IHSGEKPYEC NECGKAFSQS SDLTKHQRIH TGEKPYECSE CGKAFNRNSY LILHRRIHTR 300
EKPYKCTKCG KAFTRSSTLT LHHRIHARER ASEYSPASLD AFGAFLKSCV 350 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro. 
Interpro
 IPR001909; Krueppel-associated_box.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF01352; KRAB 
SMART
 SM00349; KRAB
 SM00355; ZnF_C2H2 
PROSITE
 PS50805; KRAB
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS