UniProt Accession

 EFTS_MYCS2; A0QVB9; I7G8N4 

Genbank Protein ID

 CP000480; CP001663 

Genbank Nucleotide ID

 ABK70376.1; AFP38929.1 

Protein Name

 Elongation factor Ts 

Protein Synonyms/Alias

 EF-Ts 

Gene Name

 tsf 

Gene Synonyms/Alias

 MSMEG_2520; MSMEI_2461 

Created Date

 July 27, 2013 

Organism

 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 

NCBI Taxa ID

 246196 

Lysine Modification

Position	Peptide	Type	References
36	EADGDFDKAVELLRI	pupylation	[1, 2]
111	IETLKAAKTGDTTVE	pupylation	[2]
131	LSAKIGEKLELRRAA	pupylation	[2]

Reference

 [1] Expansion of the mycobacterial "PUPylome".
 Watrous J, Burns K, Liu WT, Patel A, Hook V, Bafna V, Barry CE 3rd, Bark S, Dorrestein PC.
 Mol Biosyst. 2010 Feb;6(2):376-85. [PMID: 20094657]
 [2] Proteome-wide identification of mycobacterial pupylation targets.
 Poulsen C, Akhter Y, Jeon AH, Schmitt-Ulms G, Meyer HE, Stefanski A, Stühler K, Wilmanns M, Song YH.
 Mol Syst Biol. 2010 Jul 13;6:386. [PMID: 20631680] 

Functional Description

 Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome (By similarity). 

Sequence Annotation

 REGION 76 79 Involved in Mg(2+) ion dislocation from
 CROSSLNK 36 36 Isoglutamyl lysine isopeptide (Lys-Gln)  

Keyword

 Complete proteome; Cytoplasm; Elongation factor; Isopeptide bond; Protein biosynthesis; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 275 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0003746; F:translation elongation factor activity; IEA:HAMAP. 

Interpro

 IPR001816; Transl_elong_EFTs/EF1B.
 IPR014039; Transl_elong_EFTs/EF1B_dimer.
 IPR018101; Transl_elong_Ts_CS.
 IPR009060; UBA-like.
 IPR000449; UBA/transl_elong_EF1B_N. 

Pfam

 PF00889; EF_TS
 PF00627; UBA 

SMART

  

PROSITE

 PS01126; EF_TS_1
 PS01127; EF_TS_2 

PRINTS