UniProt Accession

 FBX5_HUMAN; Q9UKT4; B3KNX5; Q5TF47; Q8WV29; Q9UGC8 

Genbank Protein ID

 AF129535; AY079515; AK055221; AL080276; AL080276; CH471051; BC018905 

Genbank Nucleotide ID

 AAF04469.1; AAL86610.1; BAG51487.1; CAI18894.1; CAI18895.1; EAW47719.1; AAH18905.1 

Protein Name

 F-box only protein 5 

Protein Synonyms/Alias

 Early mitotic inhibitor 1 

Gene Name

 FBXO5 

Gene Synonyms/Alias

 EMI1; FBX5 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
64	HSGLKLVKPDDIGRL	ubiquitination	[1]
88	GSCKDCIKDYERLSC	ubiquitination	[1]
238	LQNIIGRKMGLECVD	ubiquitination	[1]
297	GAFQLYSKAIQRVTE	ubiquitination	[1]
344	LKKDAQTKLSNQGDQ	ubiquitination	[1]
352	LSNQGDQKGSTYSRH	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding. 

Sequence Annotation

 DOMAIN 250 296 F-box.
 ZN_FING 357 419 IBR-type.
 REGION 135 244 Interaction with EVI5.  

Keyword

 3D-structure; Alternative splicing; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Metal-binding; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 447 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005819; C:spindle; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; TAS:Reactome.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0060565; P:inhibition of mitotic anaphase-promoting complex activity; IDA:UniProtKB.
 GO:0007091; P:metaphase/anaphase transition of mitotic cell cycle; TAS:Reactome.
 GO:0046785; P:microtubule polymerization; IEA:Compara.
 GO:0001556; P:oocyte maturation; IEA:Compara.
 GO:0051437; P:positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
 GO:0040020; P:regulation of meiosis; IEA:Compara.
 GO:0000083; P:regulation of transcription involved in G1/S phase of mitotic cell cycle; TAS:Reactome.
 GO:0007057; P:spindle assembly involved in female meiosis I; IEA:Compara.
 GO:0016050; P:vesicle organization; IEA:Compara. 

Interpro

 IPR001810; F-box_dom_cyclin-like. 

Pfam

 PF00646; F-box 

SMART

  

PROSITE

 PS50181; FBOX 

PRINTS