CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-033011
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Tubulin alpha-8 chain 
Protein Synonyms/Alias
  
Gene Name
 TUBA8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
376DWCPTGFKVGINYQPubiquitination[1, 2]
394VPGGDLAKVQRAVCMubiquitination[1, 2]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). 
Sequence Annotation
  
Keyword
 Complete proteome; Cytoplasm; Cytoskeleton; GTP-binding; Microtubule; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 467 AA 
Protein Sequence
MLWGCRAKAC WGPRLSDLVA SLSPQRECIS VHVGQAGVQI GNACWELFCL EHGIQADGTF 60
DAQASKINDD DSFTTFFSET GNGKHVPRAV MIDLEPTVVD EVRAGTYRQL FHPEQLITGK 120
EDAANNYARG HYTVGKESID LVLDRIRKLT DACSGLQGFL IFHSFGGGTG SGFTSLLMER 180
LSLDYGKKSK LEFAIYPAPQ VSTAVVEPYN SILTTHTTLE HSDCAFMVDN EAIYDICRRN 240
LDIERPTYTN LNRLISQIVS SITASLRFDG ALNVDLTEFQ TNLVPYPRIH FPLVTYAPII 300
SAEKAYHEQL SVAEITSSCF EPNSQMVKCD PRHGKYMACC MLYRGDVVPK DVNVAIAAIK 360
TKRTIQFVDW CPTGFKVGIN YQPPTVVPGG DLAKVQRAVC MLSNTTAIAE AWARLDHKFD 420
LMYAKRAFVH WYVGEGMEEG EFSEAREDLA ALEKDYEEVG TDSFEEE 467 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0005200; F:structural constituent of cytoskeleton; IEA:InterPro.
 GO:0007017; P:microtubule-based process; IEA:InterPro.
 GO:0051258; P:protein polymerization; IEA:InterPro. 
Interpro
 IPR002452; Alpha_tubulin.
 IPR008280; Tub_FtsZ_C.
 IPR000217; Tubulin.
 IPR018316; Tubulin/FtsZ_2-layer-sand-dom.
 IPR023123; Tubulin_C.
 IPR017975; Tubulin_CS.
 IPR003008; Tubulin_FtsZ_GTPase. 
Pfam
 PF00091; Tubulin
 PF03953; Tubulin_C 
SMART
 SM00864; Tubulin
 SM00865; Tubulin_C 
PROSITE
 PS00227; TUBULIN 
PRINTS
 PR01162; ALPHATUBULIN.
 PR01161; TUBULIN.