CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007901
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 T-complex protein 1 subunit theta 
Protein Synonyms/Alias
 TCP-1-theta; CCT-theta 
Gene Name
 CCT8 
Gene Synonyms/Alias
 YJL008C; J1374 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
15NPNAGLFKQGYNSYSubiquitination[1, 2, 3, 4]
322RYGILVLKVPSKFELacetylation[5]
326LVLKVPSKFELRRLCacetylation[5]
372GDRVTVFKQEQGEISubiquitination[4]
Reference
 [1] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [2] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [4] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [5] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. In yeast may play a role in mitotic spindle formation (By similarity). 
Sequence Annotation
 MOD_RES 505 505 Phosphoserine.
 CROSSLNK 15 15 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; ATP-binding; Chaperone; Complete proteome; Cytoplasm; Isopeptide bond; Nucleotide-binding; Phosphoprotein; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 568 AA 
Protein Sequence
MSLRLPQNPN AGLFKQGYNS YSNADGQIIK SIAAIRELHQ MCLTSMGPCG RNKIIVNHLG 60
KIIITNDAAT MLRELDIVHP AVKVLVMATE QQKIDMGDGT NLVMILAGEL LNVSEKLISM 120
GLSAVEIIQG YNMARKFTLK ELDEMVVGEI TDKNDKNELL KMIKPVISSK KYGSEDILSE 180
LVSEAVSHVL PVAQQAGEIP YFNVDSIRVV KIMGGSLSNS TVIKGMVFNR EPEGHVKSLS 240
EDKKHKVAVF TCPLDIANTE TKGTVLLHNA QEMLDFSKGE EKQIDAMMKE IADMGVECIV 300
AGAGVGELAL HYLNRYGILV LKVPSKFELR RLCRVCGATP LPRLGAPTPE ELGLVETVKT 360
MEIGGDRVTV FKQEQGEISR TSTIILRGAT QNNLDDIERA IDDGVAAVKG LMKPSGGKLL 420
PGAGATEIEL ISRITKYGER TPGLLQLAIK QFAVAFEVVP RTLAETAGLD VNEVLPNLYA 480
AHNVTEPGAV KTDHLYKGVD IDGESDEGVK DIREENIYDM LATKKFAINV ATEAATTVLS 540
IDQIIMAKKA GGPRAPQGPR PGNWDQED 568 
Gene Ontology
 GO:0005832; C:chaperonin-containing T-complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0051082; F:unfolded protein binding; IDA:SGD.
 GO:0006457; P:protein folding; IDA:SGD. 
Interpro
 IPR012721; Chap_CCT_theta.
 IPR017998; Chaperone_TCP-1.
 IPR002194; Chaperonin_TCP-1_CS.
 IPR002423; Cpn60/TCP-1.
 IPR027409; GroEL-like_apical_dom.
 IPR027413; GROEL-like_equatorial.
 IPR027410; TCP-1-like_intermed. 
Pfam
 PF00118; Cpn60_TCP1 
SMART
  
PROSITE
 PS00750; TCP1_1
 PS00751; TCP1_2
 PS00995; TCP1_3 
PRINTS
 PR00304; TCOMPLEXTCP1.