CPLM-013845

Genbank Nucleotide ID

Serine/threonine-protein kinase BRSK1

Protein Synonyms/Alias

Brain-specific serine/threonine-protein kinase 1; BR serine/threonine-protein kinase 1; Serine/threonine-protein kinase SAD-B

Mus musculus (Mouse)

Position	Peptide	Type	References
42	RLEKTLGKGQTGLVK	ubiquitination	[1]
167	ENLLLDEKNNIRIAD	ubiquitination	[1]

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]

Functional Description

Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-504' and 'Ser-554'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma- tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.

DOMAIN 34 285 Protein kinase.
DOMAIN 314 356 UBA.
NP_BIND 40 48 ATP (By similarity).
ACT_SITE 156 156 Proton acceptor (By similarity).
BINDING 63 63 ATP (Probable).
MOD_RES 189 189 Phosphothreonine; by LKB1.
MOD_RES 447 447 Phosphoserine (By similarity).
MOD_RES 508 508 Phosphoserine (By similarity).
MOD_RES 563 563 Phosphoserine (By similarity).

Alternative splicing; ATP-binding; Cell cycle; Cell junction; Complete proteome; Cytoplasm; Cytoskeleton; DNA damage; Kinase; Magnesium; Metal-binding; Neurogenesis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Synapse; Transferase.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0030054; C:cell junction; IEA:UniProtKB-KW.
GO:0005813; C:centrosome; IDA:UniProtKB.
GO:0005634; C:nucleus; ISS:UniProtKB.
GO:0005886; C:plasma membrane; IEA:Compara.
GO:0008021; C:synaptic vesicle; ISS:UniProtKB.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0043015; F:gamma-tubulin binding; IDA:UniProtKB.
GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
GO:0050321; F:tau-protein kinase activity; IDA:UniProtKB.
GO:0007409; P:axonogenesis; IMP:UniProtKB.
GO:0051298; P:centrosome duplication; IMP:UniProtKB.
GO:0030010; P:establishment of cell polarity; IMP:UniProtKB.
GO:0031572; P:G2 DNA damage checkpoint; ISS:UniProtKB.
GO:0007269; P:neurotransmitter secretion; ISS:UniProtKB.
GO:0009411; P:response to UV; ISS:UniProtKB.

IPR011009; Kinase-like_dom.
IPR000719; Prot_kinase_cat_dom.
IPR017441; Protein_kinase_ATP_BS.
IPR002290; Ser/Thr_dual-sp_kinase_dom.
IPR008271; Ser/Thr_kinase_AS.
IPR015940; UBA/transl_elong_EF1B_N_euk.

PS00107; PROTEIN_KINASE_ATP
PS50011; PROTEIN_KINASE_DOM
PS00108; PROTEIN_KINASE_ST
PS50030; UBA