CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042556
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 E3 ubiquitin-protein ligase 
Protein Synonyms/Alias
  
Gene Name
 NEDD4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
230ESRRTQWKRPTPQDNubiquitination[1, 2]
465TWEDPRLKIPAHLRGubiquitination[1]
473IPAHLRGKTSLDTSNubiquitination[1, 2, 3, 4]
507FYINHNIKRTQWEDPubiquitination[1, 4, 5]
552KQNDIPNKFEMKLRRubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Ligase; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 910 AA 
Protein Sequence
XNGDCKPEMS EIKLNSDSEY IKLMHRTSAC LPSSQNVDCQ ININGELERP HSQMNKNHGI 60
LRRSISLGGA YPNISCLSSL KHNCSKGGPS QLLIKFASGN EGKVDNLSRD SNRDCTNELS 120
NSCKTENPRL ERPYTFKDFV LHPRSHKSRV KGYLRLKMTY LPKTSGSEDD NAEQAEELEP 180
GWVVLDQPDA ACHLQQQQEP SPLPPGWEER QDILGRTYYV NHESRRTQWK RPTPQDNLTD 240
AENGNIQLQA QRAFTTRRQI SEETESVDNR ESSENWEIIR EDEATMYSNQ AFPSPPPSSN 300
LDVPTHLAEE LNARLTIFGN SAVSQPASSS NHSSRRGSLQ AYTFEEQPTL PVLLPTSSGL 360
PPGWEEKQDE RGRSYYVDHN SRTTTWTKPT VQATVETSQL TSSQSSAGPQ SQASTSDSGQ 420
QVTQPSEIEQ GFLPKGWEVR HAPNGRPFFI DHNTKTTTWE DPRLKIPAHL RGKTSLDTSN 480
DLGPLPPGWE ERTHTDGRIF YINHNIKRTQ WEDPRLENVA ITGPAVPYSR DYKRKYEFFR 540
RKLKKQNDIP NKFEMKLRRA TVLEDSYRRI MGVKRADFLK ARLWIEFDGE KGLDYGGVAR 600
EWFFLISKEM FNPYYGLFEY SATDNYTLQI NPNSGLCNED HLSYFKFIGR VAGMAVYHGK 660
LLDGFFIRPF YKMMLHKPIT LHDMESVDSE YYNSLRWILE NDPTELDLRF IIDEELFGQT 720
HQHELKNGGS EIVVTNKNKK EYIYLVIQWR FVNRIQKQMA AFKEGFFELI PQDLIKIFDE 780
NELELLMCGL GDVDVNDWRE HTKYKNGYSA NHQVIQWFWK AVLMMDSEKR IRLLQFVTGT 840
SRVPMNGFAE LYGSNGPQSF TVEQWGTPEK LPRAHTCFNR LDLPPYESFE ELWDKLQMAI 900
ENTQGFDGVD 910 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro. 
Interpro
 IPR024928; E3_ub_ligase_SMURF1.
 IPR000569; HECT.
 IPR001202; WW_dom. 
Pfam
 PF00632; HECT
 PF00397; WW 
SMART
 SM00119; HECTc
 SM00456; WW 
PROSITE
 PS50237; HECT
 PS01159; WW_DOMAIN_1
 PS50020; WW_DOMAIN_2 
PRINTS