CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021613
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Intraflagellar transport protein 122 homolog 
Protein Synonyms/Alias
 WD repeat-containing protein 10; WD repeat-containing protein 140 
Gene Name
 IFT122 
Gene Synonyms/Alias
 SPG; WDR10; WDR140 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10AVLTWRDKAEHCINDubiquitination[1]
21CINDIAFKPDGTQLIubiquitination[1]
50GTLLQPLKGHKDTVYubiquitination[1]
53LQPLKGHKDTVYCVAubiquitination[1]
63VYCVAYAKDGLWSPEubiquitination[1]
72GLWSPEQKSVSKHKSubiquitination[1]
116KNGEEKVKIERPGGSubiquitination[1]
151AVADWGQKVSFYQLSubiquitination[1]
160SFYQLSGKQIGKDRAubiquitination[1]
180CCISYFTKGEYILLGubiquitination[1]
191ILLGGSDKQVSLFTKubiquitination[1]
198KQVSLFTKDGVRLGTubiquitination[1]
271QHLITEQKVRIKCKEubiquitination[1]
352CLSFSGVKEREWQMEubiquitination[1]
366ESLIRYIKVIGGPPGubiquitination[1]
417DMSASRKKLAVVDENubiquitination[1]
468GGGYLNIKASTFPVHubiquitination[1]
520YLDRKLFKEAYQIACubiquitination[1]
552GLDFETAKKAFIRVQubiquitination[1]
553LDFETAKKAFIRVQDubiquitination[1]
594DVFSYQGKFHEAAKLubiquitination[1]
600GKFHEAAKLYKRSGHubiquitination[1]
634FLGSGDPKETKMLITubiquitination[1]
637SGDPKETKMLITKQAubiquitination[1]
642ETKMLITKQADWARNubiquitination[1]
706LCATYLKKLDSPGYAubiquitination[1]
719YAAETYLKMGDLKSLubiquitination[1]
776EAQKAFHKAGRQREAubiquitination[1]
877FLLHSLPKDTPSGISubiquitination[1]
885DTPSGISKVKILFTLubiquitination[1]
887PSGISKVKILFTLAKubiquitination[1]
894KILFTLAKQSKALGAubiquitination[1]
897FTLAKQSKALGAYRLubiquitination[1]
911LARHAYDKLRGLYIPubiquitination[1]
923YIPARFQKSIELGTLubiquitination[1]
935GTLTIRAKPFHDSEEubiquitination[1]
1031ILRLVETKDSIGDEDubiquitination[1]
1074SRRDVLIKRWPPPLRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Required for cilia formation during neuronal patterning. Acts as a negative regulator of Shh signaling. Required to recruit TULP3 to primary cilia (By similarity). 
Sequence Annotation
 REPEAT 10 50 WD 1.
 REPEAT 51 91 WD 2.
 REPEAT 93 129 WD 3.
 REPEAT 131 169 WD 4.
 REPEAT 278 317 WD 5.
 REPEAT 319 359 WD 6.
 REPEAT 512 551 WD 7.  
Keyword
 Alternative splicing; Cell projection; Ciliopathy; Cilium; Cilium biogenesis/degradation; Complete proteome; Cytoplasm; Cytoskeleton; Developmental protein; Disease mutation; Ectodermal dysplasia; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1241 AA 
Protein Sequence
MRAVLTWRDK AEHCINDIAF KPDGTQLILA AGSRLLVYDT SDGTLLQPLK GHKDTVYCVA 60
YAKDGLWSPE QKSVSKHKSS SKIICCSWTN DGQYLALGMF NGIISIRNKN GEEKVKIERP 120
GGSLSPIWSI CWNPSREERN DILAVADWGQ KVSFYQLSGK QIGKDRALNF DPCCISYFTK 180
GEYILLGGSD KQVSLFTKDG VRLGTVGEQN SWVWTCQAKP DSNYVVVGCQ DGTISFYQLI 240
FSTVHGLYKD RYAYRDSMTD VIVQHLITEQ KVRIKCKELV KKIAIYRNRL AIQLPEKILI 300
YELYSEDLSD MHYRVKEKII KKFECNLLVV CANHIILCQE KRLQCLSFSG VKEREWQMES 360
LIRYIKVIGG PPGREGLLVG LKNGQILKIF VDNLFAIVLL KQATAVRCLD MSASRKKLAV 420
VDENDTCLVY DIDTKELLFQ EPNANSVAWN TQCEDMLCFS GGGYLNIKAS TFPVHRQKLQ 480
GFVVGYNGSK IFCLHVFSIS AVEVPQSAPM YQYLDRKLFK EAYQIACLGV TDTDWRELAM 540
EALEGLDFET AKKAFIRVQD LRYLELISSI EERKKRGETN NDLFLADVFS YQGKFHEAAK 600
LYKRSGHENL ALEMYTDLCM FEYAKDFLGS GDPKETKMLI TKQADWARNI KEPKAAVEMY 660
ISAGEHVKAI EICGDHGWVD MLIDIARKLD KAEREPLLLC ATYLKKLDSP GYAAETYLKM 720
GDLKSLVQLH VETQRWDEAF ALGEKHPEFK DDIYMPYAQW LAENDRFEEA QKAFHKAGRQ 780
REAVQVLEQL TNNAVAESRF NDAAYYYWML SMQCLDIAQA DPAQKDTMLG KFYHFQRLAE 840
LYHGYHAIHR HTEDPFSVHR PETLFNISRF LLHSLPKDTP SGISKVKILF TLAKQSKALG 900
AYRLARHAYD KLRGLYIPAR FQKSIELGTL TIRAKPFHDS EELVPLCYRC STNNPLLNNL 960
GNVCINCRQP FIFSASSYDV LHLVEFYLEE GITDEEAISL IDLEVLRPKR DDRQLEIANN 1020
SSQILRLVET KDSIGDEDPF TAKLSFEQGG SEFVPVVVSR LVLRSMSRRD VLIKRWPPPL 1080
RWQYFRSLLP DASITMCPSC FQMFHSEDYE LLVLQHGCCP YCRRCKDDPG P 1131 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0030991; C:intraflagellar transport particle A; IDA:UniProtKB.
 GO:0005932; C:microtubule basal body; IEA:UniProtKB-SubCell.
 GO:0032391; C:photoreceptor connecting cilium; ISS:UniProtKB.
 GO:0048593; P:camera-type eye morphogenesis; ISS:UniProtKB.
 GO:0060830; P:ciliary receptor clustering involved in smoothened signaling pathway; IEA:Compara.
 GO:0060271; P:cilium morphogenesis; ISS:UniProtKB.
 GO:0010172; P:embryonic body morphogenesis; ISS:UniProtKB.
 GO:0042733; P:embryonic digit morphogenesis; IEA:Compara.
 GO:0035050; P:embryonic heart tube development; ISS:UniProtKB.
 GO:0035721; P:intraflagellar retrograde transport; ISS:UniProtKB.
 GO:0060173; P:limb development; ISS:UniProtKB.
 GO:0045879; P:negative regulation of smoothened signaling pathway; ISS:UniProtKB.
 GO:0001843; P:neural tube closure; ISS:UniProtKB.
 GO:0060831; P:smoothened signaling pathway involved in dorsal/ventral neural tube patterning; IEA:Compara. 
Interpro
 IPR011990; TPR-like_helical.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS