UniProt Accession

 MTU1_MOUSE; Q9DAT5 

Genbank Protein ID

 AY349617; AK005541; AK043532; AK044059; BC061026 

Genbank Nucleotide ID

 AAQ55218.1; BAB24110.1; BAC31570.1; BAC31758.1; AAH61026.1 

Protein Name

 Mitochondrial tRNA-specific 2-thiouridylase 1 

Protein Synonyms/Alias

  

Gene Name

 Trmu 

Gene Synonyms/Alias

 Mtu1; Trmt1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
106	INCNKHIKFSCFYHY	acetylation	[1, 2]

Reference

 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2- thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base (By similarity). 

Sequence Annotation

 NP_BIND 10 17 ATP (By similarity).
 REGION 96 98 Interaction with target base in tRNA (By
 REGION 171 173 Interaction with tRNA (By similarity).
 REGION 334 335 Interaction with tRNA (By similarity).
 ACT_SITE 101 101 Nucleophile (By similarity).
 ACT_SITE 222 222 Cysteine persulfide intermediate (By
 BINDING 36 36 ATP; via amide nitrogen and carbonyl
 BINDING 126 126 ATP; via amide nitrogen (By similarity).
 DISULFID 101 222 Alternate (By similarity).  

Keyword

 ATP-binding; Complete proteome; Disulfide bond; Mitochondrion; Nucleotide-binding; Reference proteome; RNA-binding; Transferase; tRNA processing; tRNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 417 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016783; F:sulfurtransferase activity; IEA:InterPro.
 GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
 GO:0008033; P:tRNA processing; IEA:UniProtKB-KW. 

Interpro

 IPR023382; Adenine_a_hdrlase_dom.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR004506; tRNA-specific_2-thiouridylase. 

Pfam

 PF03054; tRNA_Me_trans 

SMART

  

PROSITE

  

PRINTS