CPLM-010553

Genbank Nucleotide ID

Rho-related GTP-binding protein RhoG

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
130	PDTLRRLKEQGQAPI	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
147	QQGQALAKQIHAVRY	ubiquitination	[1, 4, 5, 8]
183	VLNPTPIKRGRSCIL	ubiquitination	[1, 2, 4, 5, 6, 7, 8]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]

Functional Description

Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.

NP_BIND 10 17 GTP (By similarity).
NP_BIND 57 61 GTP (By similarity).
NP_BIND 115 118 GTP (By similarity).
MOTIF 32 40 Effector region (Potential).
MOD_RES 39 39 ADP-ribosylasparagine; by botulinum toxin
MOD_RES 188 188 Cysteine methyl ester (By similarity).
LIPID 188 188 S-geranylgeranyl cysteine (By

ADP-ribosylation; Cell membrane; Complete proteome; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Prenylation; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0005886; C:plasma membrane; TAS:Reactome.
GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
GO:0003924; F:GTPase activity; IDA:MGI.
GO:0030036; P:actin cytoskeleton organization; IDA:MGI.
GO:0032863; P:activation of Rac GTPase activity; IMP:UniProtKB.
GO:0007411; P:axon guidance; TAS:Reactome.
GO:0060326; P:cell chemotaxis; IMP:UniProtKB.
GO:0030168; P:platelet activation; TAS:Reactome.
GO:0008284; P:positive regulation of cell proliferation; TAS:ProtInc.
GO:0090004; P:positive regulation of establishment of protein localization to plasma membrane; IMP:UniProtKB.
GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:MGI.
GO:0016601; P:Rac protein signal transduction; IDA:MGI.
GO:0007266; P:Rho protein signal transduction; IDA:MGI.

IPR027417; P-loop_NTPase.
IPR005225; Small_GTP-bd_dom.
IPR001806; Small_GTPase.
IPR003578; Small_GTPase_Rho.

PR00449; RASTRNSFRMNG.