CPLM-009599

Genbank Nucleotide ID

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Protein Synonyms/Alias

Transducin beta chain 1

Homo sapiens (Human)

Position	Peptide	Type	References
15	RQEAEQLKNQIRDAR	ubiquitination	[1, 2, 3]
23	NQIRDARKACADATL	ubiquitination	[2, 3, 4]
57	TLRGHLAKIYAMHWG	ubiquitination	[4, 5, 6]
89	WDSYTTNKVHAIPLR	ubiquitination	[3, 4, 5, 6]
209	GACDASAKLWDVREG	ubiquitination	[2, 3, 7, 8]
280	ITSVSFSKSGRLLLA	ubiquitination	[2, 3, 7, 8, 9, 10]
301	CNVWDALKADRAGVL	ubiquitination	[1, 2, 3, 4, 7, 9]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[9] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[10] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]

Functional Description

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.

REPEAT 53 83 WD 1.
REPEAT 95 125 WD 2.
REPEAT 141 170 WD 3.
REPEAT 182 212 WD 4.
REPEAT 224 254 WD 5.
REPEAT 268 298 WD 6.
REPEAT 310 340 WD 7.
MOD_RES 2 2 N-acetylserine.
MOD_RES 266 266 Phosphohistidine (By similarity).

3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Phosphoprotein; Reference proteome; Repeat; Transducer; WD repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0044297; C:cell body; IEA:Compara.
GO:0030425; C:dendrite; IEA:Compara.
GO:0005834; C:heterotrimeric G-protein complex; IDA:MGI.
GO:0001917; C:photoreceptor inner segment; IEA:Compara.
GO:0042622; C:photoreceptor outer segment membrane; IEA:Compara.
GO:0003924; F:GTPase activity; IDA:MGI.
GO:0004871; F:signal transducer activity; IEA:UniProtKB-KW.
GO:0007191; P:adenylate cyclase-activating dopamine receptor signaling pathway; ISS:BHF-UCL.
GO:0010659; P:cardiac muscle cell apoptotic process; IEA:Compara.
GO:0008283; P:cell proliferation; IEA:Compara.
GO:0071870; P:cellular response to catecholamine stimulus; ISS:BHF-UCL.
GO:0071377; P:cellular response to glucagon stimulus; TAS:Reactome.
GO:0071456; P:cellular response to hypoxia; IEA:Compara.
GO:0071380; P:cellular response to prostaglandin E stimulus; ISS:BHF-UCL.
GO:0007204; P:elevation of cytosolic calcium ion concentration; IEA:Compara.
GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
GO:0007213; P:G-protein coupled acetylcholine receptor signaling pathway; TAS:ProtInc.
GO:0007200; P:phospholipase C-activating G-protein coupled receptor signaling pathway; IEA:Compara.
GO:0030168; P:platelet activation; TAS:Reactome.
GO:0007265; P:Ras protein signal transduction; TAS:ProtInc.
GO:0060041; P:retina development in camera-type eye; IEA:Compara.
GO:0050909; P:sensory perception of taste; IEA:Compara.
GO:0007268; P:synaptic transmission; TAS:Reactome.

IPR020472; G-protein_beta_WD-40_rep.
IPR001632; Gprotein_B.
IPR016346; Guanine_nucleotide-bd_bsu.
IPR015943; WD40/YVTN_repeat-like_dom.
IPR001680; WD40_repeat.
IPR019775; WD40_repeat_CS.
IPR017986; WD40_repeat_dom.

PS00678; WD_REPEATS_1
PS50082; WD_REPEATS_2
PS50294; WD_REPEATS_REGION

PR00319; GPROTEINB.
PR00320; GPROTEINBRPT.