CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019638
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Spermatid perinuclear RNA-binding protein 
Protein Synonyms/Alias
  
Gene Name
 STRBP 
Gene Synonyms/Alias
 SPNR 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
122ETLLNTVKDNLPIQIubiquitination[1]
216QARANGLKSCVIVLRubiquitination[1, 2]
335MDPLPSSKPFQKYSWubiquitination[1]
339PSSKPFQKYSWSVTDubiquitination[3, 4]
347YSWSVTDKEGAGSSAubiquitination[1]
384LRKILDSKAIDLMNAubiquitination[1]
526NEKRRGLKYELISETubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Involved in spermatogenesis and sperm function. Plays a role in regulation of cell growth. Binds to double-stranded DNA and RNA. Binds most efficiently to poly(I:C) RNA than to poly(dI:dC) DNA. Binds also to single-stranded poly(G) RNA. Binds non-specifically to the mRNA PRM1 3'-UTR and adenovirus VA RNA (By similarity). 
Sequence Annotation
 DOMAIN 87 334 DZF.
 DOMAIN 387 453 DRBM 1.
 DOMAIN 510 576 DRBM 2.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Developmental protein; Differentiation; DNA-binding; Polymorphism; Reference proteome; Repeat; RNA-binding; Spermatogenesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 672 AA 
Protein Sequence
MRSIRSFAND DRHVMVKHST IYPSPEELEA VQNMVSTVEC ALKHVSDWLD ETNKGTKTEG 60
ETEVKKDEAG ENYSKDQGGR TLCGVMRIGL VAKGLLIKDD MDLELVLMCK DKPTETLLNT 120
VKDNLPIQIQ KLTEEKYQVE QCVNEASIII RNTKEPTLTL KVILTSPLIR DELEKKDGEN 180
VSMKDPPDLL DRQKCLNALA SLRHAKWFQA RANGLKSCVI VLRILRDLCN RVPTWAPLKG 240
WPLELICEKS IGTCNRPLGA GEALRRVMEC LASGILLPGG PGLHDPCERD PTDALSYMTI 300
QQKEDITHSA QHALRLSAFG QIYKVLEMDP LPSSKPFQKY SWSVTDKEGA GSSALKRPFE 360
DGLGDDKDPN KKMKRNLRKI LDSKAIDLMN ALMRLNQIRP GLQYKLLSQS GPVHAPVFTM 420
SVDVDGTTYE ASGPSKKTAK LHVAVKVLQA MGYPTGFDAD IECMSSDEKS DNESKNETVS 480
SNSSNNTGNS TTETSSTLEV RTQGPILTAS GKNPVMELNE KRRGLKYELI SETGGSHDKR 540
FVMEVEVDGQ KFRGAGPNKK VAKASAALAA LEKLFSGPNA ANNKKKKIIP QAKGVVNTAV 600
SAAVQAVRGR GRGTLTRGAF VGATAAPGYI APGYGTPYGY STAAPAYGLP KRMVLLPVMK 660
FPTYPVPHYS FF 672 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0015630; C:microtubule cytoskeleton; IEA:Compara.
 GO:0005634; C:nucleus; IDA:LIFEdb.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003725; F:double-stranded RNA binding; IEA:Compara.
 GO:0003727; F:single-stranded RNA binding; IEA:Compara.
 GO:0006928; P:cellular component movement; IEA:Compara.
 GO:0007638; P:mechanosensory behavior; IEA:Compara.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0007286; P:spermatid development; IEA:Compara. 
Interpro
 IPR001159; Ds-RNA-bd.
 IPR014720; dsRNA-bd-like_dom.
 IPR006561; DZF. 
Pfam
 PF00035; dsrm
 PF07528; DZF 
SMART
 SM00358; DSRM
 SM00572; DZF 
PROSITE
 PS50137; DS_RBD 
PRINTS