CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006815
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex 
Protein Synonyms/Alias
 Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase 
Gene Name
 bfmBB 
Gene Synonyms/Alias
 bfmB; bfmB2; BSU24030 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
322KDITGLAKKVRDGKLacetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 
Functional Description
 The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). 
Sequence Annotation
 DOMAIN 1 77 Lipoyl-binding.
 ACT_SITE 395 395 Potential.
 ACT_SITE 399 399 Potential.
 MOD_RES 44 44 N6-lipoyllysine (Potential).  
Keyword
 Acyltransferase; Complete proteome; Glycolysis; Lipoyl; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 424 AA 
Protein Sequence
MAIEQMTMPQ LGESVTEGTI SKWLVAPGDK VNKYDPIAEV MTDKVNAEVP SSFTGTITEL 60
VGEEGQTLQV GEMICKIETE GANPAEQKQE QPAASEAAEN PVAKSAGAAD QPNKKRYSPA 120
VLRLAGEHGI DLDQVTGTGA GGRITRKDIQ RLIETGGVQE QNPEELKTAA PAPKSASKPE 180
PKEETSYPAS AAGDKEIPVT GVRKAIASNM KRSKTEIPHA WTMMEVDVTN MVAYRNSIKD 240
SFKKTEGFNL TFFAFFVKAV AQALKEFPQM NSMWAGDKII QKKDINISIA VATEDSLFVP 300
VIKNADEKTI KGIAKDITGL AKKVRDGKLT ADDMQGGTFT VNNTGSFGSV QSMGIINYPQ 360
AAILQVESIV KRPVVMDNGM IAVRDMVNLC LSLDHRVLDG LVCGRFLGRV KQILESIDEK 420
TSVY 424 
Gene Ontology
 GO:0043754; F:dihydrolipoyllysine-residue (2-methylpropanoyl)transferase activity; IEA:EC.
 GO:0006096; P:glycolysis; IEA:UniProtKB-KW. 
Interpro
 IPR003016; 2-oxoA_DH_lipoyl-BS.
 IPR001078; 2-oxoacid_DH_actylTfrase.
 IPR000089; Biotin_lipoyl.
 IPR023213; CAT-like_dom.
 IPR004167; E3-bd.
 IPR011053; Single_hybrid_motif. 
Pfam
 PF00198; 2-oxoacid_dh
 PF00364; Biotin_lipoyl
 PF02817; E3_binding 
SMART
  
PROSITE
 PS50968; BIOTINYL_LIPOYL
 PS00189; LIPOYL 
PRINTS