UniProt Accession

 BRCC3_HUMAN; P46736; A6QRF8; A6QRF9; A8MUX5; A8MWH0; A9Z1Y0; A9Z1Y5; B1B062; B4DQN7; Q16107; Q53YX5; Q9BTZ6 

Genbank Protein ID

 X64643; S68015; AY438030; AK298886; AK299194; AK313544; BX293995; BX470111; BX293995; BX470111; BX470111; BX293995; BX470111; BX293995; BX470111; BX470111; BX470111; BX293995; BX470111; BX293995; BX293995; BX470111; BX293995; BX470111; BC002999; BC006540 

Genbank Nucleotide ID

 CAA45917.1; AAB29005.2; AAR30498.1; BAG60999.1; BAG61237.1; BAG36320.1; CAH70537.1; CAH70537.1; CAH70538.1; CAH70538.1; CAI41654.1; CAI41654.1; CAI41655.1; CAI41655.1; CAO03573.1; CAO03574.1; CAO03575.1; CAO03575.1; CAO03576.1; CAO03576.1; CAO03601.1; CAO03601.1; CAO03602.1; CAO03602.1; AAH02999.1; AAH06540.1 

Protein Name

 Lys-63-specific deubiquitinase BRCC36 

Protein Synonyms/Alias

 BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36 

Gene Name

 BRCC3 

Gene Synonyms/Alias

 BRCC36; C6.1A; CXorf53 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
51	DDTRSDSKFAYTGTE	ubiquitination	[1, 2, 3, 4]
65	EMRTVAEKVDAVRIV	ubiquitination	[2, 3]
180	FQSIQAQKSSESLHG	ubiquitination	[1, 3]
204	HISIEGQKEEERYER	ubiquitination	[3]
226	VPHVTIGKVCLESAV	ubiquitination	[3]
237	ESAVELPKILCQEEQ	ubiquitination	[3]
261	THLDSVTKIHNGSVF	ubiquitination	[3, 5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Metalloprotease that specifically cleaves 'Lys-63'- linked polyubiquitin chains. Does not have activity toward 'Lys- 48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double- strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'- specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex. 

Sequence Annotation

 DOMAIN 7 148 MPN.
 MOTIF 122 135 JAMM motif.
 METAL 122 122 Zinc; catalytic (Probable).
 METAL 124 124 Zinc; catalytic (Probable).
 METAL 135 135 Zinc; catalytic (By similarity).
 MOD_RES 2 2 N-acetylalanine.  

Keyword

 Acetylation; Alternative splicing; Chromatin regulator; Chromosomal rearrangement; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; Hydrolase; Metal-binding; Metalloprotease; Nucleus; Polymorphism; Protease; Proto-oncogene; Reference proteome; Ubl conjugation pathway; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 316 AA 

Protein Sequence

Gene Ontology

 GO:0070531; C:BRCA1-A complex; IDA:UniProtKB.
 GO:0070552; C:BRISC complex; IDA:UniProtKB.
 GO:0000152; C:nuclear ubiquitin ligase complex; IDA:UniProtKB.
 GO:0030234; F:enzyme regulator activity; IDA:MGI.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008237; F:metallopeptidase activity; IMP:UniProtKB.
 GO:0031593; F:polyubiquitin binding; IDA:UniProtKB.
 GO:0004221; F:ubiquitin thiolesterase activity; IMP:UniProtKB.
 GO:0004843; F:ubiquitin-specific protease activity; IMP:UniProtKB.
 GO:0006302; P:double-strand break repair; IMP:UniProtKB.
 GO:0031572; P:G2 DNA damage checkpoint; IMP:UniProtKB.
 GO:0070537; P:histone H2A K63-linked deubiquitination; IDA:UniProtKB.
 GO:0045739; P:positive regulation of DNA repair; IMP:UniProtKB.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0010165; P:response to X-ray; IDA:MGI. 

Interpro

 IPR000555; JAB1_Mov34_MPN_PAD1. 

Pfam

 PF01398; JAB 

SMART

 SM00232; JAB_MPN 

PROSITE

  

PRINTS