UniProt Accession

 GSHB_RAT; P46413 

Genbank Protein ID

 L38615; BC078700 

Genbank Nucleotide ID

 AAA64618.1; AAH78700.1 

Protein Name

 Glutathione synthetase 

Protein Synonyms/Alias

 GSH synthetase; GSH-S; Glutathione synthase 

Gene Name

 Gss 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
108	ARLFDIYKQVLKEGI	acetylation	[1]
112	DIYKQVLKEGIAQTV	acetylation	[1]
138	QCSADGSKALKQIEI	acetylation	[1]
172	HVLNVLNKTNEASKI	acetylation	[1]
178	NKTNEASKILSNNPS	acetylation	[1]
186	ILSNNPSKGLALGIA	acetylation	[1]
231	ENELLDRKIHVIRRR	acetylation	[1]
443	GTTLVMNKHVGHLLR	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

  

Sequence Annotation

 NP_BIND 364 373 ATP (By similarity).
 NP_BIND 398 401 ATP (By similarity).
 REGION 148 151 Substrate binding (By similarity).
 REGION 214 216 Substrate binding (By similarity).
 REGION 267 270 Substrate binding (By similarity).
 REGION 461 462 Substrate binding (By similarity).
 METAL 144 144 Magnesium (By similarity).
 METAL 146 146 Magnesium (By similarity).
 METAL 368 368 Magnesium (By similarity).
 BINDING 125 125 Substrate (By similarity).
 BINDING 144 144 ATP (By similarity).
 BINDING 220 220 Substrate (By similarity).
 BINDING 305 305 ATP (By similarity).
 BINDING 375 375 ATP (By similarity).
 BINDING 425 425 ATP (By similarity).
 BINDING 450 450 Substrate (By similarity).
 BINDING 452 452 ATP (By similarity).
 BINDING 458 458 ATP; via carbonyl oxygen (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Direct protein sequencing; Glutathione biosynthesis; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 474 AA 

Protein Sequence

Gene Ontology

 GO:0005524; F:ATP binding; ISS:UniProtKB.
 GO:0043295; F:glutathione binding; ISS:UniProtKB.
 GO:0004363; F:glutathione synthase activity; IDA:RGD.
 GO:0016594; F:glycine binding; IDA:RGD.
 GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0007568; P:aging; IEP:RGD.
 GO:0043200; P:response to amino acid stimulus; IEP:RGD.
 GO:0046686; P:response to cadmium ion; IEA:Compara.
 GO:0031667; P:response to nutrient levels; IEP:RGD.
 GO:0034612; P:response to tumor necrosis factor; IEP:RGD.
 GO:0009410; P:response to xenobiotic stimulus; IEP:RGD. 

Interpro

 IPR004887; Glutathione_synth_subst-bd_euk.
 IPR014042; Glutathione_synthase_a-hlx_euk.
 IPR014709; Glutathione_synthase_dom.
 IPR005615; Glutathione_synthase_euk.
 IPR014049; Glutathione_synthase_N_euk.
 IPR016185; PreATP-grasp_dom. 

Pfam

 PF03917; GSH_synth_ATP
 PF03199; GSH_synthase 

SMART

  

PROSITE

  

PRINTS