UniProt Accession

 CHCH3_HUMAN; Q9NX63 

Genbank Protein ID

 AK000427; BC011596; BC014839 

Genbank Nucleotide ID

 BAA91157.1; AAH11596.1; AAH14839.1 

Protein Name

 Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial 

Protein Synonyms/Alias

  

Gene Name

 CHCHD3 

Gene Synonyms/Alias

 MINOS3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
63	SVSDEELKRRVAEEL	ubiquitination	[1]
136	EKDRVLKKQDAFYKE	ubiquitination	[1]
142	KKQDAFYKEQLARLE	acetylation	[2]
142	KKQDAFYKEQLARLE	ubiquitination	[1]
165	VTTEQYQKAAEEVEA	ubiquitination	[1]
189	VCADLQAKILQCYRE	ubiquitination	[1, 3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Required for maintenance of mitochondrial crista integrity and mitochondrial function. May act as a scaffolding protein that stabilizes protein complexes involved in crista architecture and protein import. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. 

Sequence Annotation

 DOMAIN 176 217 CHCH.
 MOD_RES 49 49 Phosphotyrosine.
 MOD_RES 50 50 Phosphoserine.
 MOD_RES 58 58 Phosphoserine (By similarity).
 MOD_RES 142 142 N6-acetyllysine.
 LIPID 2 2 N-myristoyl glycine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Lipoprotein; Membrane; Mitochondrion; Mitochondrion inner membrane; Myristate; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 227 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0019902; F:phosphatase binding; IDA:UniProtKB.
 GO:0032947; F:protein complex scaffold; ISS:UniProtKB.
 GO:0001227; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; IDA:UniProtKB.
 GO:0007007; P:inner mitochondrial membrane organization; IMP:UniProtKB.
 GO:0008053; P:mitochondrial fusion; IMP:UniProtKB. 

Interpro

 IPR007964; DUF737. 

Pfam

 PF05300; DUF737 

SMART

  

PROSITE

  

PRINTS