UniProt Accession

 PPIF_RAT; P29117 

Genbank Protein ID

 U68544; BC086977 

Genbank Nucleotide ID

 AAB08453.1; AAH86977.1 

Protein Name

 Peptidyl-prolyl cis-trans isomerase F, mitochondrial 

Protein Synonyms/Alias

 PPIase F; Cyclophilin D; CyP-D; CypD; Cyclophilin F; Rotamase F 

Gene Name

 Ppif 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
66	GRVVLELKADVVPKT	acetylation	[1]
85	RALCTGEKGFGYKGS	acetylation	[1]
90	GEKGFGYKGSTFHRV	acetylation	[1]
166	KTDWLDGKHVVFGHV	acetylation	[1]
174	HVVFGHVKEGMDVVK	acetylation	[1]
181	KEGMDVVKKIESFGS	acetylation	[1]
182	EGMDVVKKIESFGSK	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probablity of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress- induced necrosis. Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis. 

Sequence Annotation

 DOMAIN 48 204 PPIase cyclophilin-type.
 MOD_RES 166 166 N6-acetyllysine (By similarity).
 MOD_RES 202 202 S-nitrosocysteine (By similarity).  

Keyword

 Acetylation; Apoptosis; Complete proteome; Cyclosporin; Direct protein sequencing; Isomerase; Mitochondrion; Necrosis; Reference proteome; Rotamase; S-nitrosylation; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 206 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IDA:RGD.
 GO:0005759; C:mitochondrial matrix; IDA:RGD.
 GO:0016018; F:cyclosporin A binding; IDA:RGD.
 GO:0003755; F:peptidyl-prolyl cis-trans isomerase activity; IDA:RGD.
 GO:0008637; P:apoptotic mitochondrial changes; IEA:Compara.
 GO:0071243; P:cellular response to arsenic-containing substance; ISS:UniProtKB.
 GO:0071277; P:cellular response to calcium ion; ISS:UniProtKB.
 GO:0070301; P:cellular response to hydrogen peroxide; ISS:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
 GO:0032780; P:negative regulation of ATPase activity; ISS:UniProtKB.
 GO:2001243; P:negative regulation of intrinsic apoptotic signaling pathway; ISS:UniProtKB.
 GO:0090324; P:negative regulation of oxidative phosphorylation; ISS:UniProtKB.
 GO:2000276; P:negative regulation of oxidative phosphorylation uncoupler activity; ISS:UniProtKB.
 GO:0090201; P:negative regulation of release of cytochrome c from mitochondria; ISS:UniProtKB.
 GO:0010940; P:positive regulation of necrotic cell death; ISS:UniProtKB.
 GO:0090200; P:positive regulation of release of cytochrome c from mitochondria; ISS:UniProtKB.
 GO:0006457; P:protein folding; IEA:UniProtKB-KW.
 GO:0046902; P:regulation of mitochondrial membrane permeability; IDA:RGD.
 GO:0010849; P:regulation of proton-transporting ATPase activity, rotational mechanism; ISS:UniProtKB.
 GO:0002931; P:response to ischemia; ISS:UniProtKB. 

Interpro

 IPR002130; Cyclophilin-like_PPIase_dom.
 IPR024936; Cyclophilin-type_PPIase.
 IPR020892; Cyclophilin-type_PPIase_CS. 

Pfam

 PF00160; Pro_isomerase 

SMART

  

PROSITE

 PS00170; CSA_PPIASE_1
 PS50072; CSA_PPIASE_2 

PRINTS

 PR00153; CSAPPISMRASE.