CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019739
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein SCAF11 
Protein Synonyms/Alias
 CTD-associated SR protein 11; Renal carcinoma antigen NY-REN-40; SC35-interacting protein 1; SR-related and CTD-associated factor 11; SRSF2-interacting protein; Serine/arginine-rich splicing factor 2-interacting protein; Splicing factor, arginine/serine-rich 2-interacting protein; Splicing regulatory protein 129; SRrp129 
Gene Name
 SCAF11 
Gene Synonyms/Alias
 CASP11; SFRS2IP; SIP1; SRSF2IP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
83ASCPIDRKPFQAVFKubiquitination[1]
112QLRETKDKKNENSFEubiquitination[2]
113LRETKDKKNENSFEKubiquitination[3]
120KNENSFEKQVSCHENubiquitination[1, 2, 4]
129VSCHENSKSCIRRKAubiquitination[2, 4]
135SKSCIRRKAIVREDLubiquitination[1]
146REDLLSAKVCDLKWIubiquitination[1, 2, 4]
151SAKVCDLKWIHRNSLubiquitination[1, 4]
165LYSETGGKKNAAIKIubiquitination[2, 3]
166YSETGGKKNAAIKINubiquitination[1]
171GKKNAAIKINKPQRSubiquitination[1]
285EHFGTSCKGYALAHTubiquitination[1]
298HTQEGEEKKQTSGTSubiquitination[3]
299TQEGEEKKQTSGTSNubiquitination[1]
1090YRSSFAYKDQNENRWubiquitination[1, 5]
1124FVEQQSYKRKSEQEFubiquitination[5]
1126EQQSYKRKSEQEFSFubiquitination[1]
1152ASSWAVRKTLPADVQubiquitination[1, 5, 6, 7]
1178GPQSGWMKQEEETSGubiquitination[3]
1392QEVKLAIKPFYQNKDacetylation[8]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861
Functional Description
 Plays a role in pre-mRNA alternative splicing by regulating spliceosome assembly. 
Sequence Annotation
 ZN_FING 41 82 RING-type; degenerate.
 MOD_RES 338 338 Phosphoserine.
 MOD_RES 341 341 Phosphoserine.
 MOD_RES 344 344 Phosphoserine.
 MOD_RES 400 400 Phosphoserine.
 MOD_RES 401 401 Phosphoserine.
 MOD_RES 402 402 Phosphoserine.
 MOD_RES 405 405 Phosphoserine.
 MOD_RES 410 410 Phosphothreonine.
 MOD_RES 413 413 Phosphoserine.
 MOD_RES 533 533 Phosphoserine.
 MOD_RES 608 608 Phosphoserine.
 MOD_RES 614 614 Phosphoserine.
 MOD_RES 680 680 Phosphoserine.
 MOD_RES 687 687 Phosphoserine.
 MOD_RES 723 723 Phosphoserine.
 MOD_RES 726 726 Phosphoserine.
 MOD_RES 769 769 Phosphothreonine.
 MOD_RES 771 771 Phosphoserine.
 MOD_RES 776 776 Phosphoserine.
 MOD_RES 796 796 Phosphoserine.
 MOD_RES 798 798 Phosphoserine.
 MOD_RES 802 802 Phosphoserine.
 MOD_RES 807 807 Phosphothreonine.
 MOD_RES 963 963 Phosphoserine.
 MOD_RES 1127 1127 Phosphoserine.
 MOD_RES 1169 1169 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Metal-binding; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1463 AA 
Protein Sequence
MKKKTVCTLN MGDKKYEDME GEENGDNTIS TGLLYSEADR CPICLNCLLE KEVGFPESCN 60
HVFCMTCILK WAETLASCPI DRKPFQAVFK FSALEGYVKV QVKKQLRETK DKKNENSFEK 120
QVSCHENSKS CIRRKAIVRE DLLSAKVCDL KWIHRNSLYS ETGGKKNAAI KINKPQRSNW 180
STNQCFRNFF SNMFSSVSHS GESSFTYRAY CTEFIEASEI SALIRQKRHE LELSWFPDTL 240
PGIGRIGFIP WNVETEVLPL ISSVLPRTIF PTSTISFEHF GTSCKGYALA HTQEGEEKKQ 300
TSGTSNTRGS RRKPAMTTPT RRSTRNTRAE TASQSQRSPI SDNSGCDAPG NSNPSLSVPS 360
SAESEKQTRQ APKRKSVRRG RKPPLLKKKL RSSVAAPEKS SSNDSVDEET AESDTSPVLE 420
KEHQPDVDSS NICTVQTHVE NQSANCLKSC NEQIEESEKH TANYDTEERV GSSSSESCAQ 480
DLPVLVGEEG EVKKLENTGI EANVLCLESE ISENILEKGG DPLEKQDQIS GLSQSEVKTD 540
VCTVHLPNDF PTCLTSESKV YQPVSCPLSD LSENVESVVN EEKITESSLV EITEHKDFTL 600
KTEELIESPK LESSEGEIIQ TVDRQSVKSP EVQLLGHVET EDVEIIATCD TFGNEDFNNI 660
QDSENNLLKN NLLNTKLEKS LEEKNESLTE HPRSTELPKT HIEQIQKHFS EDNNEMIPME 720
CDSFCSDQNE SEVEPSVNAD LKQMNENSVT HCSENNMPSS DLADEKVETV SQPSESPKDT 780
IDKTKKPRTR RSRFHSPSTT WSPNKDTPQE KKRPQSPSPR RETGKESRKS QSPSPKNESA 840
RGRKKSRSQS PKKDIARERR QSQSRSPKRD TTRESRRSES LSPRRETSRE NKRSQPRVKD 900
SSPGEKSRSQ SRERESDRDG QRRERERRTR KWSRSRSHSR SPSRCRTKSK SSSFGRIDRD 960
SYSPRWKGRW ANDGWRCPRG NDRYRKNDPE KQNENTRKEK NDIHLDADDP NSADKHRNDC 1020
PNWITEKINS GPDPRTRNPE KLKESHWEEN RNENSGNSWN KNFGSGWVSN RGRGRGNRGR 1080
GTYRSSFAYK DQNENRWQNR KPLSGNSNSS GSESFKFVEQ QSYKRKSEQE FSFDTPADRS 1140
GWTSASSWAV RKTLPADVQN YYSRRGRNSS GPQSGWMKQE EETSGQDSSL KDQTNQQVDG 1200
SQLPINMMQP QMNVMQQQMN AQHQPMNIFP YPVGVHAPLM NIQRNPFNIH PQLPLHLHTG 1260
VPLMQVATPT SVSQGLPPPP PPPPPSQQVN YIASQPDGKQ LQGIPSSSHV SNNMSTPVLP 1320
APTAAPGNTG MVQGPSSGNT SSSSHSKASN AAVKLAESKV SVAVEASADS SKTDKKLQIQ 1380
EKAAQEVKLA IKPFYQNKDI TKEEYKEIVR KAVDKVCHSK SGEVNSTKVA NLVKAYVDKY 1440
KYSRKGSQKK TLEEPVSTEK NIG 1463 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0000245; P:spliceosomal complex assembly; IDA:UniProtKB. 
Interpro
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 
Pfam
 PF13639; zf-RING_2 
SMART
 SM00184; RING 
PROSITE
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS