CPLM-014671

Genbank Nucleotide ID

Metalloreductase STEAP3

Protein Synonyms/Alias

Dudulin-2; Six-transmembrane epithelial antigen of prostate 3; Tumor suppressor-activated pathway protein 6; hTSAP6; pHyde; hpHyde

Homo sapiens (Human)

Position	Peptide	Type	References
339	DLVNLAVKQVLANKS	ubiquitination	[1]
472	WERESTIKFTLPTDH	ubiquitination	[1, 2]
484	TDHALAEKTSHV***	ubiquitination	[2, 3, 4, 5, 6]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[3] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]

Functional Description

Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.

DOMAIN 259 407 Ferric oxidoreductase.
METAL 316 316 Iron (heme axial ligand) (Probable).
METAL 409 409 Iron (heme axial ligand) (Probable).
BINDING 36 36 NADP.
BINDING 38 38 NADP; via amide nitrogen.
BINDING 39 39 NADP; via amide nitrogen.
BINDING 58 58 NADP.
BINDING 59 59 NADP.
BINDING 91 91 NADP; via carbonyl oxygen.
BINDING 116 116 NADP; via amide nitrogen.
BINDING 151 151 NADP; via amide nitrogen.
MOD_RES 17 17 Phosphoserine (By similarity).
MOD_RES 20 20 Phosphoserine (By similarity).
CARBOHYD 256 256 N-linked (GlcNAc...) (Probable).
CARBOHYD 344 344 N-linked (GlcNAc...) (Probable).

3D-structure; Alternative splicing; Apoptosis; Cell cycle; Complete proteome; Copper; Endosome; FAD; Flavoprotein; Glycoprotein; Ion transport; Iron; Iron transport; Membrane; Metal-binding; NADP; Oxidoreductase; Phosphoprotein; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0010008; C:endosome membrane; TAS:Reactome.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005771; C:multivesicular body; IDA:MGI.
GO:0005886; C:plasma membrane; IEA:Compara.
GO:0000293; F:ferric-chelate reductase activity; IEA:Compara.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
GO:0006879; P:cellular iron ion homeostasis; TAS:Reactome.
GO:0006917; P:induction of apoptosis; IEA:Compara.
GO:0009306; P:protein secretion; IMP:UniProtKB.
GO:0033572; P:transferrin transport; TAS:Reactome.
GO:0055085; P:transmembrane transport; TAS:Reactome.

IPR013130; Fe3_Rdtase_TM_dom.
IPR016040; NAD(P)-bd_dom.
IPR004455; NADP_OxRdtase_F420.

PF03807; F420_oxidored
PF01794; Ferric_reduct