CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020422
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NACHT, LRR and PYD domains-containing protein 1 
Protein Synonyms/Alias
 Caspase recruitment domain-containing protein 7; Death effector filament-forming ced-4-like apoptosis protein; Nucleotide-binding domain and caspase recruitment domain 
Gene Name
 NLRP1 
Gene Synonyms/Alias
 CARD7; DEFCAP; KIAA0926; NAC; NALP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
297RSQDPLVKRSWPDYVubiquitination[1]
340QGAAGIGKSTLARQVubiquitination[1]
855GLTAEDCKDLAFGLRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Able to form cytoplasmic structures termed death effector filaments. Enhances APAF1 and cytochrome c-dependent activation of pro-caspase-9 and consecutive apoptosis. Stimulates apoptosis through activation of caspase-3. Involved in activation of caspase-1 and caspase-5 as part of the NALP1 inflammasome complex which leads to processing and release of IL1B and IL18. Binds ATP. 
Sequence Annotation
 DOMAIN 1 92 DAPIN.
 DOMAIN 328 637 NACHT.
 REPEAT 809 830 LRR 1.
 REPEAT 838 858 LRR 2.
 REPEAT 866 887 LRR 3.
 REPEAT 895 915 LRR 4.
 REPEAT 923 944 LRR 5.
 REPEAT 950 973 LRR 6.
 DOMAIN 1374 1463 CARD.
 NP_BIND 334 341 ATP (Potential).  
Keyword
 3D-structure; Alternative splicing; Apoptosis; ATP-binding; Complete proteome; Cytoplasm; Leucine-rich repeat; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1473 AA 
Protein Sequence
MAGGAWGRLA CYLEFLKKEE LKEFQLLLAN KAHSRSSSGE TPAQPEKTSG MEVASYLVAQ 60
YGEQRAWDLA LHTWEQMGLR SLCAQAQEGA GHSPSFPYSP SEPHLGSPSQ PTSTAVLMPW 120
IHELPAGCTQ GSERRVLRQL PDTSGRRWRE ISASLLYQAL PSSPDHESPS QESPNAPTST 180
AVLGSWGSPP QPSLAPREQE APGTQWPLDE TSGIYYTEIR EREREKSEKG RPPWAAVVGT 240
PPQAHTSLQP HHHPWEPSVR ESLCSTWPWK NEDFNQKFTQ LLLLQRPHPR SQDPLVKRSW 300
PDYVEENRGH LIEIRDLFGP GLDTQEPRIV ILQGAAGIGK STLARQVKEA WGRGQLYGDR 360
FQHVFYFSCR ELAQSKVVSL AELIGKDGTA TPAPIRQILS RPERLLFILD GVDEPGWVLQ 420
EPSSELCLHW SQPQPADALL GSLLGKTILP EASFLITART TALQNLIPSL EQARWVEVLG 480
FSESSRKEYF YRYFTDERQA IRAFRLVKSN KELWALCLVP WVSWLACTCL MQQMKRKEKL 540
TLTSKTTTTL CLHYLAQALQ AQPLGPQLRD LCSLAAEGIW QKKTLFSPDD LRKHGLDGAI 600
ISTFLKMGIL QEHPIPLSYS FIHLCFQEFF AAMSYVLEDE KGRGKHSNCI IDLEKTLEAY 660
GIHGLFGAST TRFLLGLLSD EGEREMENIF HCRLSQGRNL MQWVPSLQLL LQPHSLESLH 720
CLYETRNKTF LTQVMAHFEE MGMCVETDME LLVCTFCIKF SRHVKKLQLI EGRQHRSTWS 780
PTMVVLFRWV PVTDAYWQIL FSVLKVTRNL KELDLSGNSL SHSAVKSLCK TLRRPRCLLE 840
TLRLAGCGLT AEDCKDLAFG LRANQTLTEL DLSFNVLTDA GAKHLCQRLR QPSCKLQRLQ 900
LVSCGLTSDC CQDLASVLSA SPSLKELDLQ QNNLDDVGVR LLCEGLRHPA CKLIRLGLDQ 960
TTLSDEMRQE LRALEQEKPQ LLIFSRRKPS VMTPTEGLDT GEMSNSTSSL KRQRLGSERA 1020
ASHVAQANLK LLDVSKIFPI AEIAEESSPE VVPVELLCVP SPASQGDLHT KPLGTDDDFW 1080
GPTGPVATEV VDKEKNLYRV HFPVAGSYRW PNTGLCFVMR EAVTVEIEFC VWDQFLGEIN 1140
PQHSWMVAGP LLDIKAEPGA VEAVHLPHFV ALQGGHVDTS LFQMAHFKEE GMLLEKPARV 1200
ELHHIVLENP SFSPLGVLLK MIHNALRFIP VTSVVLLYHR VHPEEVTFHL YLIPSDCSIR 1260
KAIDDLEMKF QFVRIHKPPP LTPLYMGCRY TVSGSGSGML EILPKELELC YRSPGEDQLF 1320
SEFYVGHLGS GIRLQVKDKK DETLVWEALV KPGDLMPATT LIPPARIAVP SPLDAPQLLH 1380
FVDQYREQLI ARVTSVEVVL DKLHGQVLSQ EQYERVLAEN TRPSQMRKLF SLSQSWDRKC 1440
KDGLYQALKE THPHLIMELW EKGSKKGLLP LSS 1473 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0072558; C:NLRP1 inflammasome complex; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:BHF-UCL.
 GO:0005524; F:ATP binding; IDA:HGNC.
 GO:0008656; F:cysteine-type endopeptidase activator activity involved in apoptotic process; NAS:UniProtKB.
 GO:0042742; P:defense response to bacterium; ISS:BHF-UCL.
 GO:0006917; P:induction of apoptosis; NAS:UniProtKB.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0051402; P:neuron apoptotic process; IDA:HGNC.
 GO:0035872; P:nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway; TAS:Reactome.
 GO:0050718; P:positive regulation of interleukin-1 beta secretion; ISS:BHF-UCL.
 GO:0050727; P:regulation of inflammatory response; IC:BHF-UCL.
 GO:0032495; P:response to muramyl dipeptide; ISS:BHF-UCL. 
Interpro
 IPR001315; CARD.
 IPR004020; DAPIN.
 IPR011029; DEATH-like_dom.
 IPR000767; Disease_R.
 IPR025307; FIIND_dom.
 IPR001611; Leu-rich_rpt.
 IPR007111; NACHT_NTPase.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00619; CARD
 PF13553; FIIND
 PF02758; PYRIN 
SMART
  
PROSITE
 PS50209; CARD
 PS50824; DAPIN
 PS51450; LRR
 PS50837; NACHT 
PRINTS
 PR00364; DISEASERSIST.