CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006426
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATP-dependent helicase SGS1 
Protein Synonyms/Alias
 Helicase TPS1 
Gene Name
 SGS1 
Gene Synonyms/Alias
 TPS1; YMR190C; YM9646.02C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
621PSWSPKIKREKSSVSsumoylation[1]
831YKELKFFKREYPDIPsumoylation[1]
Reference
 [1] Sumoylation of the BLM ortholog, Sgs1, promotes telomere-telomere recombination in budding yeast.
 Lu CY, Tsai CH, Brill SJ, Teng SC.
 Nucleic Acids Res. 2010 Jan;38(2):488-98. [PMID: 19906698] 
Functional Description
 Interacts with topoisomerases II and TOP3. Could create a deleterious topological substrate that TOP3 preferentially resolves. The TOP3-SGS1 protein complex may function as a eukaryotic reverse gyrase introducing positive supercoils into extrachromosomal ribosomal DNA rings. Together with topoisomerase II has a role in chromosomal segregation. Maintains rDNA structure where it has a role in re-starting stalled replication forks. 
Sequence Annotation
 DOMAIN 687 864 Helicase ATP-binding.
 DOMAIN 886 1035 Helicase C-terminal.
 DOMAIN 1272 1351 HRDC.
 NP_BIND 714 721 ATP (By similarity).
 MOTIF 808 811 DEAH box.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1447 AA 
Protein Sequence
MVTKPSHNLR REHKWLKETA TLQEDKDFVF QAIQKHIANK RPKTNSPPTT PSKDECGPGT 60
TNFITSIPAS GPTNTATKQH EVMQTLSNDT EWLSYTATSN QYADVPMVDI PASTSVVSNP 120
RTPNGSKTHN FNTFRPHMAS SLVENDSSRN LGSRNNNKSV IDNSSIGKQL ENDIKLEVIR 180
LQGSLIMALK EQSKLLLQKC SIIESTSLSE DAKRLQLSRD IRPQLSNMSI RIDSLEKEII 240
KAKKDGMSKD QSKGRSQVSS QDDNIISSIL PSPLEYNTSS RNSNLTSTTA TTVTKALAIT 300
GAKQNITNNT GKNSNNDSNN DDLIQVLDDE DDIDCDPPVI LKEGAPHSPA FPHLHMTSEE 360
QDELTRRRNM RSREPVNYRI PDRDDPFDYV MGKSLRDDYP DVEREEDELT MEAEDDAHSS 420
YMTTRDEEKE ENELLNQSDF DFVVNDDLDP TQDTDYHDNM DVSANIQESS QEGDTRSTIT 480
LSQNKNVQVI LSSPTAQSVP SNGQNQIGVE HIDLLEDDLE KDAILDDSMS FSFGRQHMPM 540
SHSDLELIDS EKENEDFEED NNNNGIEYLS DSDLERFDEE RENRTQVADI QELDNDLKII 600
TERKLTGDNE HPPPSWSPKI KREKSSVSQK DEEDDFDDDF SLSDIVSKSN LSSKTNGPTY 660
PWSDEVLYRL HEVFKLPGFR PNQLEAVNAT LQGKDVFVLM PTGGGKSLCY QLPAVVKSGK 720
THGTTIVISP LISLMQDQVE HLLNKNIKAS MFSSRGTAEQ RRQTFNLFIN GLLDLVYISP 780
EMISASEQCK RAISRLYADG KLARIVVDEA HCVSNWGHDF RPDYKELKFF KREYPDIPMI 840
ALTATASEQV RMDIIHNLEL KEPVFLKQSF NRTNLYYEVN KKTKNTIFEI CDAVKSRFKN 900
QTGIIYCHSK KSCEQTSAQM QRNGIKCAYY HAGMEPDERL SVQKAWQADE IQVICATVAF 960
GMGIDKPDVR FVYHFTVPRT LEGYYQETGR AGRDGNYSYC ITYFSFRDIR TMQTMIQKDK 1020
NLDRENKEKH LNKLQQVMAY CDNVTDCRRK LVLSYFNEDF DSKLCHKNCD NCRNSANVIN 1080
EERDVTEPAK KIVKLVESIQ NERVTIIYCQ DVFKGSRSSK IVQANHDTLE EHGIGKSMQK 1140
SEIERIFFHL ITIRVLQEYS IMNNSGFASS YVKVGPNAKK LLTGKMEIKM QFTISAPNSR 1200
PSTSSSFQAN EDNIPVIAQK STTIGGNVAA NPPRFISAKE HLRSYTYGGS TMGSSHPITL 1260
KNTSDLRSTQ ELNNLRMTYE RLRELSLNLG NRMVPPVGNF MPDSILKKMA AILPMNDSAF 1320
ATLGTVEDKY RRRFKYFKAT IADLSKKRSS EDHEKYDTIL NDEFVNRAAA SSNGIAQSTG 1380
TKSKFFGANL NEAKENEQII NQIRQSQLPK NTTSSKSGTR SISKSSKKSA NGRRGFRNYR 1440
GHYRGRK 1447 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:SGD.
 GO:0031422; C:RecQ helicase-Topo III complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0043140; F:ATP-dependent 3'-5' DNA helicase activity; IEA:InterPro.
 GO:0004003; F:ATP-dependent DNA helicase activity; IDA:SGD.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0006268; P:DNA unwinding involved in replication; IDA:SGD.
 GO:0000724; P:double-strand break repair via homologous recombination; IMP:SGD.
 GO:0031292; P:gene conversion at mating-type locus, DNA double-strand break processing; IGI:SGD.
 GO:0031573; P:intra-S DNA damage checkpoint; IMP:SGD.
 GO:0045132; P:meiotic chromosome segregation; IMP:SGD.
 GO:0000706; P:meiotic DNA double-strand break processing; IGI:SGD.
 GO:0000070; P:mitotic sister chromatid segregation; IMP:SGD.
 GO:0010947; P:negative regulation of meiotic joint molecule formation; IGI:SGD.
 GO:0001302; P:replicative cell aging; IMP:SGD.
 GO:0000722; P:telomere maintenance via recombination; IMP:SGD.
 GO:0031860; P:telomeric 3' overhang formation; IGI:SGD. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR002464; DNA/RNA_helicase_DEAH_CS.
 IPR004589; DNA_helicase_ATP-dep_RecQ.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR022758; Helicase_Sgs1.
 IPR010997; HRDC-like.
 IPR002121; HRDC_dom.
 IPR027417; P-loop_NTPase.
 IPR018982; RQC_domain.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C
 PF11408; Helicase_Sgs1
 PF09382; RQC 
SMART
 SM00487; DEXDc
 SM00490; HELICc
 SM00341; HRDC
 SM00956; RQC 
PROSITE
 PS00690; DEAH_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS50967; HRDC 
PRINTS