UniProt Accession

 HAOX2_RAT; Q07523 

Genbank Protein ID

 X67156; BC078781 

Genbank Nucleotide ID

 CAA47629.1; AAH78781.1 

Protein Name

 Hydroxyacid oxidase 2 

Protein Synonyms/Alias

 HAOX2; (S)-2-hydroxy-acid oxidase, peroxisomal; Long chain alpha-hydroxy acid oxidase; Long-chain L-2-hydroxy acid oxidase 

Gene Name

 Hao2 

Gene Synonyms/Alias

 Hao3; Haox2 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
10	LVCLADFKAHAQKQL	acetylation	[1]
19	HAQKQLSKTSWDFIE	acetylation	[1]
57	RYLRDMSKVDTRTTI	acetylation	[1]
139	KSDWDFNKQMVQRAE	acetylation	[1]
182	LEANILLKDLRALKE	acetylation	[1]
188	LKDLRALKEEKPTQS	acetylation	[1]
191	LRALKEEKPTQSVPV	acetylation	[1]
229	ILKGILTKEDAELAM	acetylation	[1]
237	EDAELAMKHNVQGIV	acetylation	[1]
271	REVVAAVKGKIEVYM	acetylation	[1]
273	VVAAVKGKIEVYMDG	acetylation	[1]
290	RTGTDVLKALALGAR	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Catalyzes the oxidation of L-alpha-hydroxy acids as well as, more slowly, that of L-alpha-amino acids. 

Sequence Annotation

 DOMAIN 2 353 FMN hydroxy acid dehydrogenase.
 NP_BIND 279 303 FMN.
 MOTIF 351 353 Microbody targeting signal (Potential).
 ACT_SITE 248 248 Proton acceptor (By similarity).
 BINDING 106 106 FMN.
 BINDING 128 128 FMN.
 BINDING 130 130 Substrate.
 BINDING 156 156 FMN.
 BINDING 165 165 Substrate.
 BINDING 224 224 FMN.
 BINDING 251 251 Substrate (By similarity).
 BINDING 303 303 FMN.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Flavoprotein; FMN; Oxidoreductase; Peroxisome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 353 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005777; C:peroxisome; IDA:HGNC.
 GO:0003973; F:(S)-2-hydroxy-acid oxidase activity; IDA:RGD.
 GO:0010181; F:FMN binding; IDA:RGD.
 GO:0052853; F:long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity; IEA:EC.
 GO:0052854; F:medium-chain-(S)-2-hydroxy-acid oxidase activity; IEA:EC.
 GO:0052852; F:very-long-chain-(S)-2-hydroxy-acid oxidase activity; IEA:EC.
 GO:0019395; P:fatty acid oxidation; IEA:Compara.
 GO:0018924; P:mandelate metabolic process; IDA:RGD.
 GO:0051260; P:protein homooligomerization; IDA:RGD. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR012133; Alpha-hydoxy_acid_DH_FMN.
 IPR000262; FMN-dep_DH.
 IPR008259; FMN_hydac_DH_AS. 

Pfam

 PF01070; FMN_dh 

SMART

  

PROSITE

 PS00557; FMN_HYDROXY_ACID_DH_1
 PS51349; FMN_HYDROXY_ACID_DH_2 

PRINTS