CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037424
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Orotate phosphoribosyltransferase 
Protein Synonyms/Alias
  
Gene Name
 UMPS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22LYDVQAFKFGDFVLKubiquitination[1]
29KFGDFVLKSGLSSPIubiquitination[1, 2]
160LDREQGGKDKLQAHGubiquitination[3]
162REQGGKDKLQAHGIRacetylation[4, 5]
162REQGGKDKLQAHGIRubiquitination[3]
178HSVCTLSKMLEILEQubiquitination[3]
187LEILEQQKKVDAETVubiquitination[3]
188EILEQQKKVDAETVGubiquitination[3]
219NGSPLSIKEAPKELSubiquitination[1, 2, 3]
223LSIKEAPKELSFGARubiquitination[3]
242RIHPVASKLLRLMQKubiquitination[3]
314FLIFEDRKFADIGNTubiquitination[1, 3]
323ADIGNTVKKQYEGKCubiquitination[1, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 342 AA 
Protein Sequence
MAVARAALGP LVTGLYDVQA FKFGDFVLKS GLSSPIYIDL RGIVSRPRLL SQVADILFQT 60
AQNAGISFDT VCGVPYTALP LATVICSTNQ IPMLIRRKET KDYGTKRLVE GTINPGETCL 120
IIEDVVTSGS SVLETVEVLQ KEGLKVTDAI VLLDREQGGK DKLQAHGIRL HSVCTLSKML 180
EILEQQKKVD AETVGRVKRF IQENVFVAAN HNGSPLSIKE APKELSFGAR AELPRIHPVA 240
SKLLRLMQKK ETNLCLSADV SLARELLQLA DALGPSICML KTHVDILNDF TLDVMKELIT 300
LAKCHEFLIF EDRKFADIGN TVKKQYEGKC IIQESARIRN PA 342 
Gene Ontology
 GO:0004588; F:orotate phosphoribosyltransferase activity; IEA:InterPro.
 GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IEA:InterPro.
 GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
 GO:0009116; P:nucleoside metabolic process; IEA:InterPro.
 GO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:InterPro. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR018089; OMPdecase_AS.
 IPR001754; OMPdeCOase_dom.
 IPR023031; OPRT.
 IPR004467; Or_phspho_trans_dom.
 IPR000836; PRibTrfase_dom.
 IPR011060; RibuloseP-bd_barrel. 
Pfam
 PF00215; OMPdecase
 PF00156; Pribosyltran 
SMART
  
PROSITE
 PS00156; OMPDECASE 
PRINTS