CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-028808
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rap guanine nucleotide exchange factor (GEF) 4, isoform CRA_b 
Protein Synonyms/Alias
 Rap guanine nucleotide exchange factor 4; cAMP-regulated guanine nucleotide exchange factor IIB 
Gene Name
 Rapgef4 
Gene Synonyms/Alias
 cAMP-GEFII; mCG_128278 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
58ITKVPSEKILRAGKIubiquitination[1]
64EKILRAGKILRIAILacetylation[2]
139QERHFQDKYLFYRFLubiquitination[1]
487PELEKIVKQISEDAKubiquitination[1]
501KAPQKKHKVLLQQFNubiquitination[1]
549VPVAASVKEVISAVAubiquitination[1]
771AYRLTAAKLEPPLIPubiquitination[1]
806DNLVNFEKMRMIANTubiquitination[1]
834PDAAQANKNHQDVRSubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Guanine-nucleotide releasing factor; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 867 AA 
Protein Sequence
MLYEKYRQYM AGLLAPPYGV METGSNNDRI PDKENTPLIE PHVPLRPAHT ITKVPSEKIL 60
RAGKILRIAI LSRAPHMIRD RKYHLKTYRQ CCVGTELVDW MIQQTSCVHS RTQAVGMWQV 120
LLEDGVLNHV DQERHFQDKY LFYRFLDDER EDAPLPTEEE KKECDEELQD TMLLLSQMGP 180
DAHMRMILRK PPGQRTVDDL EIIYDELLHI KALSHLSTTV KRELAGVLIF ESHAKGGTVL 240
FNQGEEGTSW YIILKGSVNV VIYGKGVVCT LHEGDDFGKL ALVNDAPRAA SIVLREDNCH 300
FLRVDKEDFN RILRDVEANT VRLKEHDQDV LVLEKVPAGN RAANQGNSQP QQKYTVMSGT 360
PEKILEHFLE TIRLEPSLNE ATDSVLNDFV MMHCVFMPNT QLCPALVAHY HAQPSQGTEQ 420
ERMDYALNNK RRVIRLVLQW AAMYGDLLQE DDVAMAFLEE FYVSVSDDAR MMAAFKEQLP 480
ELEKIVKQIS EDAKAPQKKH KVLLQQFNTG DERAQKRQPI RGSDEVLFKV YCIDHTYTTI 540
RVPVAASVKE VISAVADKLG SGEGLIIVKM NSGGEKVVLK SNDVSVFTTL TINGRLFACP 600
REQFDSLTPL PEQEGPTTGT VGTFELMSSK DLAYQMTTYD WELFNCVHEL ELIYHTFGRH 660
NFKKTTANLD LFLRRFNEIQ FWVVTEVCLC SQLSKRVQLL KKFIKIAAHC KEYKNLNSFF 720
AIVMGLSNVA VSRLALTWEK LPSKFKKFYA EFESLMDPSR NHRAYRLTAA KLEPPLIPFM 780
PLLIKDMTFT HEGNKTFIDN LVNFEKMRMI ANTARTVRYY RSQPFNPDAA QANKNHQDVR 840
SYVRQLNVID NQRTLSQMSH RLEPRRP 867 
Gene Ontology
 GO:0005952; C:cAMP-dependent protein kinase complex; IEA:InterPro.
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0008603; F:cAMP-dependent protein kinase regulator activity; IEA:InterPro.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:MGI.
 GO:0017156; P:calcium ion-dependent exocytosis; IDA:MGI.
 GO:0019933; P:cAMP-mediated signaling; IDA:MGI.
 GO:0030073; P:insulin secretion; IDA:MGI.
 GO:0017157; P:regulation of exocytosis; IDA:MGI.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR002373; cAMP/cGMP_kin.
 IPR018490; cNMP-bd-like.
 IPR000595; cNMP-bd_dom.
 IPR000591; DEP_dom.
 IPR000159; Ras-assoc.
 IPR000651; Ras-like_Gua-exchang_fac_N.
 IPR019804; Ras_G-nucl-exch_fac_CS.
 IPR008937; Ras_GEF.
 IPR023578; Ras_GEF_dom.
 IPR001895; RasGRF_CDC25.
 IPR014710; RmlC-like_jellyroll.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00027; cNMP_binding
 PF00610; DEP
 PF00788; RA
 PF00617; RasGEF
 PF00618; RasGEF_N 
SMART
 SM00100; cNMP
 SM00049; DEP
 SM00147; RasGEF
 SM00229; RasGEFN 
PROSITE
 PS50042; CNMP_BINDING_3
 PS50186; DEP
 PS00720; RASGEF
 PS50009; RASGEF_CAT
 PS50212; RASGEF_NTER 
PRINTS
 PR00103; CAMPKINASE.