CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014570
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative methyltransferase NSUN5C 
Protein Synonyms/Alias
 NOL1/NOP2/Sun domain family member 5C; Williams-Beuren syndrome chromosomal region 20C protein 
Gene Name
 NSUN5P2 
Gene Synonyms/Alias
 NSUN5C; WBSCR20B; WBSCR20C 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
65SHLAALLKNQGKIFAubiquitination[1, 2, 3, 4]
69ALLKNQGKIFAFDLDubiquitination[5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
 May have S-adenosyl-L-methionine-dependent methyl- transferase activity (Potential). 
Sequence Annotation
 REGION 50 56 S-adenosyl-L-methionine binding (By
 REGION 101 102 S-adenosyl-L-methionine binding (By
 ACT_SITE 175 175 Nucleophile (Potential).
 BINDING 74 74 S-adenosyl-L-methionine (By similarity).
 BINDING 79 79 S-adenosyl-L-methionine (By similarity).
 BINDING 121 121 S-adenosyl-L-methionine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Methyltransferase; Polymorphism; Reference proteome; S-adenosyl-L-methionine; Transferase; Williams-Beuren syndrome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 315 AA 
Protein Sequence
MPELLVFPAQ TDLHEHPLYR AGHLILQDRA SCLPAMLLDP RQAPMSWMPV PPQAIKTSHL 60
AALLKNQGKI FAFDLDARRL ASMATLLAWA GVSCCELAEE DFLAVSPLDP RYREVHYVLL 120
DPSCSGSGMP SRQLEEPGAG TPSPVRLHAL AGFQQRALCH ALTFPSLQRL VYSMCSLCQE 180
ENEDMVQDAL QQNPGAFRLA PALPARPHRG LSTFPGAEHC LRASPKTTLS GGFFVAVIER 240
VEMPTSASQA KASAPERTPS PAPKRKKRAK SCSRCLHTAL HIAEAPGSLL PGGKGRCLSS 300
PWKTLGPHRR QQFAF 315 
Gene Ontology
 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW. 
Interpro
 IPR001678; Fmu/NOL1/Nop2p.
 IPR023267; RCMT. 
Pfam
 PF01189; Nol1_Nop2_Fmu 
SMART
  
PROSITE
  
PRINTS
 PR02008; RCMTFAMILY.