CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043832
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 
Protein Synonyms/Alias
  
Gene Name
 USP4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
105RDNPLGMKGEIAEAYubiquitination[1]
133HVAPRMFKTQVGRFAubiquitination[2]
284VYNHRFHKIFQMDEGubiquitination[2]
327PVYFRERKSRPSSTSubiquitination[2]
352LLSVPKHKLTLESLYubiquitination[2]
371DRISRYVKQPLPDEFubiquitination[2]
470AADGKLLKLNSRSTLubiquitination[2, 3]
550KHQQATKKFDLWSLPubiquitination[4]
576YNRYWRDKLDTVVEFubiquitination[1, 2, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 621 AA 
Protein Sequence
XPKSSASPYS SVSASLIANG DSTSTCGMHS SGVSRGGSGF SASYNCQEPP SSHIQPGLCG 60
LGNLGNTCFM NSALQCLSNT APLTDYFLKD EYEAEINRDN PLGMKGEIAE AYAELIKQMW 120
SGRDAHVAPR MFKTQVGRFA PQFSGYQQQD SQELLAFLLD GLHEDLNRVV AKEAWENHRL 180
RNDSVIVDTF HGLFKSTLVC PECAKVSVTF DPFCYLTLPL PLKKDRVMEV FLVPADPHCR 240
PTQYRVTVPL MGAVSDLCEA LSRLSGIAAE NMVVADVYNH RFHKIFQMDE GLNHIMPRDD 300
IFVYEVCSTS VDGSECVTLP VYFRERKSRP SSTSSASALY GQPLLLSVPK HKLTLESLYQ 360
AVCDRISRYV KQPLPDEFGS SPLEPGACNG SRNSCEGEDE EEMEHQEEGK EQLSETEGSG 420
EDEPGNDPSE TTQKKIKGQP CPKRLFTFSL VNSYGTADIN SLAADGKLLK LNSRSTLAMD 480
WDSETRRLYY DEQESEAYEK HVSMLQPQKK KKTTVALRDC IELFTTMETL GEHDPWYCPN 540
CKKHQQATKK FDLWSLPKIL VVHLKRFSYN RYWRDKLDTV VEFPIRGLNM SEFVCNLSAR 600
PYVYDLIAVS NHYGAMGVGH Y 621 
Gene Ontology
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19. 
Pfam
 PF00443; UCH 
SMART
  
PROSITE
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS