UniProt Accession

 CDC27_BOVIN; A7Z061 

Genbank Protein ID

 BC153259 

Genbank Nucleotide ID

 AAI53260.1 

Protein Name

 Cell division cycle protein 27 homolog 

Protein Synonyms/Alias

  

Gene Name

 CDC27 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Bos taurus (Bovine) 

NCBI Taxa ID

 9913 

Lysine Modification

Position	Peptide	Type	References
84	KCCVDLSKLAEGEQI	ubiquitination	[1]
99	LSGGVFNKQKSHDDI	ubiquitination	[1, 2]
101	GGVFNKQKSHDDIVT	ubiquitination	[1]
133	CKTDRLAKGSECYQK	ubiquitination	[1]
391	TSDSSTTKENSKKLK	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity). 

Sequence Annotation

 REPEAT 84 114 TPR 1.
 REPEAT 115 148 TPR 2.
 REPEAT 500 533 TPR 3.
 REPEAT 568 601 TPR 4.
 REPEAT 603 635 TPR 5.
 REPEAT 636 669 TPR 6.
 REPEAT 671 703 TPR 7.
 REPEAT 705 737 TPR 8.
 REPEAT 738 771 TPR 9.
 MOD_RES 205 205 Phosphothreonine (By similarity).
 MOD_RES 209 209 Phosphothreonine (By similarity).
 MOD_RES 244 244 Phosphothreonine (By similarity).
 MOD_RES 291 291 Phosphoserine (By similarity).
 MOD_RES 313 313 Phosphothreonine (By similarity).
 MOD_RES 339 339 Phosphoserine (By similarity).
 MOD_RES 367 367 Phosphothreonine (By similarity).
 MOD_RES 387 387 Phosphoserine (By similarity).
 MOD_RES 427 427 Phosphoserine (By similarity).
 MOD_RES 431 431 Phosphothreonine (By similarity).
 MOD_RES 436 436 Phosphoserine (By similarity).
 MOD_RES 439 439 Phosphoserine (By similarity).
 MOD_RES 447 447 Phosphothreonine (By similarity).
 MOD_RES 822 822 Phosphoserine (By similarity).  

Keyword

 Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; TPR repeat; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 825 AA 

Protein Sequence

Gene Ontology

 GO:0005680; C:anaphase-promoting complex; ISS:UniProtKB.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; IEA:InterPro.
 GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB. 

Interpro

 IPR026803; CDC27.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR013105; TPR_2.
 IPR019734; TPR_repeat. 

Pfam

 PF00515; TPR_1
 PF07719; TPR_2 

SMART

 SM00028; TPR 

PROSITE

 PS50005; TPR
 PS50293; TPR_REGION 

PRINTS